Vitaly P. Zubov

Personal information

Vitali Zubov (Prof., PhD, Dr. Sci) is a Head of the Polymers for Biology Laboratory of the IBCh RAS. His academic career began in Lomonosov Moscow State University where he has been a Professor in Chemistry Department since 1986.

Scientific interests

His scientific interests include the synthesis and chemical conversions of polymers, the kinetics and mechanisms of radical polymerization.

Main scientific results

He is an author of 350 papers and reviews, 120 patents, and 2 books. He has developed and investigated a number of new polymer-containing materials for medical diagnostics and bioseparation, in particular using the polymerization processes in adsorbed layers of inorganic matrices. These materials (sorbents, membranes, etc.) have unique sorption properties due to the immobilization of nano-scale polymer layers on the carrier surfaces.

Selected publications

  1. Ягудаева Е.Ю., Жигис Л.С., Разгуляева О.А., Зуева В.С., Мельников Э.Э., Зубов В.П., Козлов Л.В., Бичучер А.М., Котельникова О.В., Аллилуев А.П., Аваков А.Э., Румш Л.Д. (2010). Выделение и определение активности IGA1-протеиназы из культуры Neisseria meningitidis. Биоорг. хим. 36 (1), 96–105 [+]

    A method of the isolation and purification of IgA1 protease from a culture of Neisseria meningitidis serogroup A has been developed. It was shown that IgA1 protease isolated from serogroup A meningococcus is capable of protecting experimental animals (mice) infected with meningococcus of serogroup B.

  2. Ягудаева Е.Ю., Букина Я.А., Простякова А.И., Зубов В.П., Тверской В.А., Капустин Д.В. (2009). Окислительная полимеризация анилина на поверхности кремнезема в присутствии полисульфокислот как способ получения эффективных биосорбентов. Высокомолекулярные соединения 51 (6), 1000–1007 [+]

    Polyaniline coatings on the surface of the macroporous silica have been prepared by oxidative polymerization via protonation of aniline by poly(sulfonic acids): poly(p,p'_(2,2'_disulfonic acid)diphenyleneisophthalamide (iso-PSA) and poly(p,p'_(2,2'_disulfonic acid)diphenyleneterephthalamide (tere-PSA).

  3. Ягудаева Е.Ю., Муйдинов М.Р., Капустин Д.В., Зубов В.П. (2007). Окислительная полимеризация анилина на поверхности гетерогенных поликислот как способ получения эффективных биосорбентов. Известия РАН, ОХН.  (6), 1123–1130 [+]

    Polyaniline coatings of the synthetic composite material based on silica gel surface modified by a sulfated styrene—divinylbenzene copolymer were prepared by precipitative polymerization of aniline on the surface of the supports. The optimal conditions for the preparation of the polyaniline-containing material providing the formation of a thin polymeric coating on the support surface were determined. Aniline is predominantly consumed to the formation of a uniform polymeric coating about 3 nm thick with an increase in the concentration of sulfo groups on the support surface. The efficiency of using the polyaniline-containing sorbents for the preparative isolation of DNA from plant tissue lysates was demonstrated.

  4. Zubov V.P., Kapustin D.V., Generalova A.N., Yagudaeva E.Y.u., Vikhrov A.A., Sizova S.V., Muidinov M.R. (2007). Modification of solids with polymer nanolayers as a process for manufacture of novel biomaterial. POLYMER SCIENCE SERIES A 49 (12), 1247–1264 [+]

    The results of study on the chemical deposition of polymeric coatings of a nanoscale thickness on porous and flat inorganic matrices and encapsulation of nano-and microparticles in polymer shells are discussed. Procedures for the deposition of homogeneous defect-free coatings are detailed by using polytetrafluoroethylene, polyaniline, and their derivatives as examples. The matrices modified with nanosized polytetrafluoroethylene and polyaniline layers are promising biomaterials for one-step isolation of nucleic acids from complex biological mixtures (cell and tissue lysates, whole blood, plant feedstock), as well as for high-performance chromatography of proteins and other biopolymers. Approaches to the fabrication of polymer shells on luminescent nanocrystals of (CdSe)ZnS via the inclusion of the nanocrystals in micrometer-sized particles based on acrolein-styrene copolymers and the formation of polymer shells directly on nanoparticles are discussed. It was shown that polymer-functionalized luminescent nanocrystals hold promise as bioanalytical reagents.

  5. Stashevskaya K., Markvicheva E., Strukova S., Prudchenko I., Zubov V., Grandfils C.h. (2007). Thrombin receptor agonist peptide etrapped in poly(d,l,-lactide-co-glycolide) microcapsules:preparation and characterization. J Microencapsulation 24 (2), 129–142 [+]

    Thrombin receptor agonist peptide (TRAP-6) could advantageously replace thrombin in terms of accelerating wound healing being less expensive and more stable. To promote TRAP-6 pharmacological action as a tissue reconstruction stimulator this study investigated its entrapment within poly(D,L)-lactide-co-glycolide (PLGA) microparticles. Due to its low molecular weight and water solubility, TRAP-6 microencapsulated form is expected to be more useful. This paper reports TRAP-6 microencapsulation by a double (w/o/w) emulsion-vaporation technique. TRAP-6 release kinetics were evaluated by both chemical (HPLC) and biological assays in vitro. The results revealed a high level of TRAP-6 sensitivity to physico-chemical events during the microencapsulation. The surface morphology difference between control microparticles (without TRAP-6) and microparticles with entrapped TRAP-6 during in vitro degradation highlighted a particular role of TRAP-6. The results can allow one to optimize the microencapsulation procedure and to encounter a new promising approach to development of biodegradable polymer drug delivery systems for wound healing.

  6. Kapustin D.V., Yagudaeva E.Y., Zubov V.P., Muydinov M.R., Yaroshevskaja E.M., Plobner L., Leiser R.-.M., Brem G. (2006). New Polymer-Coated Materials for One-Step Separation of Nucleic Acids. Frontiers in DNA Research , 113–136
  7. Kapustin D.V., Vikhrov A.A., Gorokhova I.V., Generalova A.N., Kalyazina O.V., Murzabekova T.V., Zubov V.P. (2005). Multicomponent thermosensitive systems for biocatalysts. RUSSIAN CHEMICAL BULLETIN 54 (2), 452–457 [+]

    Composite matrices based on macroporous silica modified by N-vinylcaprolactam copolymers with diallyldimethylammonium chloride and with 2-hydroxyethyl methacrylate were obtained. Lipase from Pseudomonas fluorescens was immobilized on the obtained materials. The temperature dependence of the hydrolytic activity of the immobilized lipase preparations in the triacetin hydrolysis was investigated. The hydrolytic activity of lipase immobilized on the matrix modified by the N-vinylcaprolactam copolymer with 2-hydroxyethyl methacrylate can be regulated by varying the temperature of the reaction medium. The temperature dependence of the hydrolytic activity of the immobilized enzyme has a maximum at 40 °C, the activity of the immobilized lipase being ∼3.5 times higher compared to that at 20 °C. After immobilization on these composite materials, lipase retained the activity in the acetylation of 1-(RS)-phenylethanol with vinyl acetate in ButOMe.

  8. Капустин Д.В., Ягудаева Е.Ю., Завада Л.Л., Жигис Л.С., Зубов В.П., Ярошевская Е.М., Плобнер Л., Лайзер Р.-.М., Брем Г. (2003). Композиционный полианилинсодержащий кремнеземный сорбент для выделения ДНК. Биоорг. хим. , 310–315 [+]

    A composite sorbent based on porous glass beads modified with thin polyaniline coating was prepared by precipitation aniline polymerization in the presence of carrier surface. It was shown that the modification ensures the uniform coating of the inner surface of the carrier pores with the polymer layer~1nm thick. It was shown that the resulting material retains the initial porosity of the carrier and is selective in the separation of nucleic acids and proteins. The polyaniline-coated sorbents were shown to be efficient for the preparative DNA isolation from bacterial lysates.

  9. Рапопорт Е.М., Некрасов М.В., Хайдуков С.В., Свирщевская Е.В., Жигис Л.С., Козлов Л.В., Баталова Т.Н., Зубов В.П., Бовин Н.В. (2000). Изучение клеточной локализации галактозосвязывающего лектина из сыворотки крови человека. Биохимия 65 (11), 1558–1563
  10. Bovin N.V., Korchagina E.Y., Zemlyanukhina T.V., Byramova N.E., Galanina O.E., Zemlyakov A.E., Ivanov A.E., Zubov V.P., Mochalova L.V. (1993). Synthesis of polymeric neoglycoconjugates based on N-substituted polyacrylamides. Glycoconj. J. 10 (2), 142–51 [+]

    Describes synthesis and application of new indispensable probes for study of carbohydrate-binding proteins both in artificial and cellular systems