J Pharm Biomed Anal, 2004, 34(1):199-206

HPLC electrospray mass spectrometric characterization of trimeric building blocks for oligonucleotide synthesis

Trimeric nucleotide building blocks are valuable in synthesis of randomized oligonucleotides. In this study, we have developed HPLC-MS and HPLC-MS/MS methods for quality control of protected trinucleotides. C18 reversed-phase HPLC was used for purity evaluation, and base sequences were verified using negative ion electrospray ionization mass spectrometry (ESI-MS) and collision induced dissociation (CID) MS/MS. The principal dissociation pathway was formation of w-ions, which represent the 3′-5′ direction. The other major fragments were d-ions, which are formed by cleavage of 5′ C-O bonds of the sugars. The developed method was suitable for verification of purity and structure of the 29 trinucleotides studied. © 2003 Elsevier B.V. All rights reserved.

Mauriala T, Auriola S, Azhayev A, Kayushin A, Korosteleva M, Miroshnikov A

IBCH: 1037
Ссылка на статью в журнале: http://linkinghub.elsevier.com/retrieve/pii/S0731708503004795
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