J Interferon Cytokine Res, 1999, 19(2):137-144

Efficient human IFN-γ expression in the mammary gland of transgenic mice

Two hybrid genes (BLG-HuIFN-γ2 and BLG-HuIFN-γ3) were constructed on the basis of sheep β-lactoglobulin (BLG) and human interferon-γ(HuIFN-γ) gene sequences. They were used to direct HuIFN-γ synthesis in the mammary gland of transgenic mice. HuIFN-γ was efficiently produced in the mammary gland of transgenic mice. BLG-HuIFN-γ2 transgenic females expressed HuIFN- γ in the milk at concentrations up to 570 mg/ml, and BLG-HuIFN-γ3 transgenic females expressed up to 350 mg/ml. All females carrying the BLG- HuIFN-γ3 gene expressed HuIFN-γ in their milk. No significant changes were observed in the HuIFNγ expression level during the lactation period. Using RT-PCR analysis, ectopic expression for both hybrid genes was found in transgenic mice. Despite ectopic expression of HuIFN-γ in transgenic mice, their development and pregnancy were normal. The heritability of the HuIFN- γ expression level in milk was demonstrated up to the F2generation. This work demonstrates that hybrid genes have the potential to develop in transgenic domestic animals producing HuIFN-γ in milk.

Lagutin OV, Dobrovolsky VN, Vinogradova TV, Kyndiakov BN, Khodarovich YM, Jenkins N, James D, Markham N, Larionov OA

IBCH: 1927
Ссылка на статью в журнале: http://www.liebertpub.com/doi/10.1089/107999099314289
Кол-во цитирований на 03.2024: 8
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