Nucleic Acids Res, 1984, 12(17):6779-6795

Site-specificity of abnormal excision: The mechanism of formation of a specialized transducing bacteriophage 5§

Molecular mechanism of the specialized transducing bacteriophage λpZac5 formation has been studied. Phage-bacterial DNA junctions in λpZac5 DNA are localized and primary structure of regions of the abnormal excisional recombination leading to the phage formation is elucidated; the crossover region proved to be comparable with the central part of attP and attB sites (the core and the adjacent tetranucleotide) in length and degree of homology. Bacterial insert in λpZac5 DNA is shown to end immediately after Z-Y spacer, the DNA not containing ZacY gene segments. The data obtained led to the conclusion of site-specific (homologous) character of abnormal excision upon formation of lambda transducing bacteriophages. Possible mechanisms of the excision are discussed. © 1984 IRL Press Limited.

Shpakovski GV, Berlin YA

IBCH: 2881
Ссылка на статью в журнале: https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/12.17.6779
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