Journal of Pharmaceutical Sciences and Research, 2018, 10(6):1545-1551

Polymorphism of cattle microsatellite complexes

The article presents the results of genotyping dairy and beef cattle population by analyzing 15 microsatellite markers. For studying dairy animals, black-and-white Holstein heifers from the herd of educational and experimental farm Krasnodarskoye of the Kuban State Agrarian University (Krasnodar, Krasnodar Krai, Russia) were used. This selection was represented by 73 heads 11-12 months old. Beef animals in the selection were provided by the Stock Company Agrocomplex n.a. N. I. Tkachev (Vyselkovsky district, Krasnodar Krai, Russia). The material was obtained from 30 purebred Aberdeen-Angus heads, including 5 cows, 15 heifers and 10 bulls. The obtained biological samples were processed for obtaining the genomic DNA of the required quality. The molecular genetic identification of animals was performed based on microsatellite DNA markers (COrDIS Cattle kit manufactured by LLC Gordis, Russia). Certificates of genetic conformity were obtained in full for all dairy and beef animals. For all groups of animals, Fis values were within the confidence interval (95%), which confirmed the absence of both inbreeding and outbreeding, i.e. the studied population had stable genetic structure. The research made it possible to determine heterozygosity of the populations (0.67 to 0.95), to confirm the absence of close and closely-related inbreeding, to predict further directions of livestock breeding, to identify each animal individually, and to form and store a common database, which allowed to generate and issue passports for the animals that complied with the requirements of world standards.

Garkovenko AV, Radchenko VV, Ilnitskaya EV, Koshchaev AG, Shchukina IV, Bakharev AA, Sukhanova SF

IBCH: 3038
Ссылка на статью в журнале: https://www.jpsr.pharmainfo.in/Documents/Volumes/vol10Issue06/jpsr10061852.pdf
Кол-во цитирований на 01.2024: 51
Данные статьи проверены модераторами 2018-06-01