Chemistry, 2014, 20(41):13234-13241

Red-Shifted Fluorescent Aminated Derivatives of a Conformationally Locked GFP Chromophore

KGaA, Weinheim. A novel class of fluorescent dyes based on conformationally locked GFP chromophore is reported. These dyes are characterized by red-shifted spectra, high fluorescence quantum yields and pH-independence in physiological pH range. The intra- and intermolecular mechanisms of radiationless deactivation of ABDI-BF2 fluorophore by selective structural locking of various conformational degrees of freedom were studied. A unique combination of solvatochromic and lipophilic properties together with "infinite" photostability (due to a dynamic exchange between free and bound dye) makes some of the novel dyes promising bioinspired tools for labeling cellular membranes, lipid drops and other organelles.

IBCH: 4390
Ссылка на статью в журнале: http://doi.wiley.com/10.1002/chem.201403678
Кол-во цитирований на 11.2023: 69
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