Selective suppression of polymerase chain reaction and its most popular applications

All righrs reserved. This chapter is devoted to methods based on suppression polymerase chain reaction (PCR) effect (cDNA library construction starting from a small amount of total RNA; suppression subtractive hybridization, SSH; ordered differential display, ODD; Marathon cDNA RACE; genome walking; variants of coincidence cloning, CC; normalization of cDNA libraries; multiplex PCR, mPCR; to in vitro cloning). Taken together, these approaches allow one to analyze complex DNA samples, from searching sequences of interest to determining complete structures of the respective genes.

IBCH: 5258
Ссылка на статью в журнале: https://doi.org/10.1007/978-1-4020-6040-32
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