RUSSIAN ACADEMY OF SCIENCES
Shemyakin & Ovchinikov Institute of Bioorganic Chemistry
Laboratory of Molecular Technologies
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General information
Professional appointments
Education
Scientific background
Publications

Sergey A. Lukyanov

GENERAL INFORMATION
Born: September 13, 1963, Moscow, Russia (USSR)
Citizenship: Russia
Business address:
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Micklukho-Maklaya 16/10, 117871, Moscow, Russia;
tel: 7(495) 336-63-88
fax: 7(495) 330-70-56
e-mail: luk@ibch.ru

PROFESSIONAL APPOINTMENTS
Head of the lab, Laboratory of Molecular Technologies (prev. Laboratory of Genes for Regeneration), Shemyakin-Ovchinnnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.

EDUCATION AND DEGREES
1985 Embryology Department, Moscow State University (Moscow, Russia.)
1994 Ph.D.
1999 Dr.Sc.
2003 Corresponding Member of the Russian Academy of Science

SCIENTIFIC BACKGROUND
Laboratory of Structure and Function of Human Genes, Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia. Supervisor - Prof. E.D. Sverdlov.
1986-1988 Research assistant.
1988-1992 Junior scientist.
1992-1994 Scientist.
1994-1998 Senior scientist.
1998- Head of Laboratory, Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia

Ph.D. thesis: "Cloning of a novel Xenopus homeobox gene XANF-1 by subtractive hybridization" - 1994. Supervisor - Prof. E.D. Sverdlov.
Dr.Sc. thesis: "Selective suppression of polymerase chain reaction" - 1999.

Area of expertise: Methods for gene searching and analysis - cDNA libraries construction, cDNA and genome subtraction; cDNA differential display, cloning of full-length cDNA, genome walking, etc.
Fluorescent proteins - searching, applications, and chromophore structures determination.

PUBLICATIONS
  1. Markvicheva KN, Bogdanova EA, Staroverov DB, Lukyanov S, Belousov VV.
    Imaging of intracellular hydrogen peroxide production with HyPer upon stimulation of HeLa cells with EGF.
    In: Redox-Mediated Signal Transduction Methods and Protocols Series: Methods in Molecular Biology, Vol. 476 Hancock, John T. (Ed.) Hardcover, 2009 ISBN: 978-1-58829-842-3

  2. Anisimova VE, Barsova EV, Bogdanova EA, Lukyanov SA, Shcheglov AS.
    Thermolabile duplex-specific nuclease.
    Biotechnol Lett, 2008; [Epub ahead of print]
    PMID: 18810329

  3. Panchin AY, Spirin SA, Lukyanov SA, Lebedev YB, Panchin YV.
    Human trash ESTs--sequences from cDNA collection that are not aligned to genome assembly.
    J Bioinform Comput Biol. 2008 Aug;6(4):759-73
    PMID: 18763741

  4. Anisimova VE, Shcheglov AS, Bogdanova EA, Rebrikov DV, Nekrasov AN, Barsova EV, Shagin DA, Lukyanov SA.
    Is crab duplex-specific nuclease a member of the Serratia family of non-specific nucleases?
    Gene. 2008 Jul 15;418(1-2):41-8
    PMID: 18514436

  5. Anisimova VE, Rebrikov DV, Shagin DA, Kozhemyako VB, Menzorova NI, Staroverov DB, Ziganshin R, Vagner LL, Rasskazov VA, Lukyanov SA, Shcheglov AS.
    Isolation, characterization and molecular cloning of duplex-specific nuclease from the hepatopancreas of the Kamchatka crab.
    BMC Biochem. 2008 May 21;9:14
    PMID: 18495036

  6. Yampolsky IV, Kislukhin AA, Amatov TT, Shcherbo D, Potapov VK, Lukyanov S, Lukyanov KA.
    Synthesis and properties of the red chromophore of the green-to-red photoconvertible fluorescent protein Kaede and its analogs.
    Bioorg Chem. 2008 Apr;36(2):96-104
    PMID: 18262585

  7. Bogdanova EA, Shagin DA, Lukyanov SA.
    Normalization of full-length enriched cDNA.
    Mol Biosyst. 2008 Mar;4(3):205-12
    PMID: 18437263

  8. Buzdin A, Lukyanov S.
    Stem-loop oligonucleotides as hybridization probes and their practical use in molecular biology and biomedicine.
    In: Nucleic Acids Hybridization: Modern Applications. Buzdin, Anton; Lukyanov, Sergey (Eds.) Hardcover, 2007; 85-96 ISBN: 978-1-4020-6039-7
    FULL TEXT

  9. Lukyanov S, Rebrikov D, Buzdin A.
    Suppression subtractive hybridization
    In: Nucleic Acids Hybridization: Modern Applications. Buzdin, Anton; Lukyanov, Sergey (Eds.) Hardcover, 2007; 53-84 ISBN: 978-1-4020-6039-7
    FULL TEXT

  10. Lukyanov S, Lukyanov K, Gurskaya N, Bogdanova E, Buzdin A.
    Selective suppression of polymerase chain reaction and its most popular applications.
    In: Nucleic Acids Hybridization: Modern Applications. Buzdin, Anton; Lukyanov, Sergey (Eds.) Hardcover, 2007; 29-51 ISBN: 978-1-4020-6039-7
    FULL TEXT

  11. Chudakov DM, Lukyanov S, Lukyanov KA.
    Tracking intracellular protein movements using photoswitchable fluorescent proteins PS-CFP2 and Dendra2.
    Nat Protoc. 2007;2(8):2024-32
    PMID: 17703215

  12. Shcherbo D, Merzlyak EM, Chepurnykh TV, Fradkov AF, Ermakova GV, Solovieva EA, Lukyanov KA, Bogdanova EA, Zaraisky AG, Lukyanov S, Chudakov DM.
    Bright far-red fluorescent protein for whole-body imaging.
    Nat Methods. 2007 Sep;4(9):741-6
    PMID: 17721542

  13. Merzlyak EM, Goedhart J, Shcherbo D, Bulina ME, Shcheglov AS, Fradkov AF, Gaintzeva A, Lukyanov KA, Lukyanov S, Gadella TW, Chudakov DM.
    Bright monomeric red fluorescent protein with an extended fluorescence lifetime.
    Nat Methods. 2007 Jul;4(7):555-7
    PMID: 17572680

  14. Souslova EA, Belousov VV, Lock J, Stromblad S, Kasparov S, Bolshakov AP, Pinelis VG, Labas YA, Lukyanov S, Mayr LM, Chudakov DM.
    Single fluorescent protein-based Ca2+ sensors with increased dynamic range.
    BMC Biotechnol. 2007 Jun 29;7(1):37
    PMID: 17603870

  15. Chudakov DM, Lukyanov S, Lukyanov KA.
    Using photoactivatable fluorescent protein Dendra2 to track protein movement.
    Biotechniques. 2007 May;42(5):553, 555, 557 passim
    PMID: 17515192

  16. Zhang L, Gurskaya NG, Merzlyak EM, Staroverov DB, Mudrik NN, Samarkina ON, Vinokurov LM, Lukyanov S, Lukyanov KA.
    Method for real-time monitoring of protein degradation at the single cell level.
    Biotechniques. 2007 Apr;42(4):446, 448, 450
    PMID: 17489230

  17. Hosp J, Tashpulatov A, Roessner U, Barsova E, Katholnigg H, Steinborn R, Melikant B, Lukyanov S, Heberle-Bors E, Touraev A.
    Transcriptional and metabolic profiles of stress-induced, embryogenic tobacco microspores.
    Plant Mol Biol. 2007 Jan;63(1):137-49
    PMID: 17016740

  18. Lukyanov KA, Chudakov DM, Fradkov AF, Labas YA, Matz MV, Lukyanov S.
    Discovery and properties of GFP-like proteins from nonbioluminescent anthozoa.
    Methods Biochem Anal. 2006;47:121-38
    PMID: 16335712

  19. Bulina ME, Lukyanov KA, Britanova OV, Onichtchouk D, Lukyanov S, Chudakov DM.
    Chromophore-assisted light inactivation (CALI) using the phototoxic fluorescent protein KillerRed.
    Nat Protoc. 2006;1(2):947-53
    PMID: 17406328

  20. Chudakov DM, Chepurnykh TV, Belousov VV, Lukyanov S, Lukyanov KA.
    Fast and precise protein tracking using repeated reversible photoactivation.
    Traffic. 2006 Oct;7(10):1304-10
    PMID: 16889652

  21. Anisimova VE, Rebrikov DV, Zhulidov PA, Staroverov DB, Lukyanov SA, Shcheglov AS.
    Renaturation, activation, and practical use of recombinant duplex-specific nuclease from Kamchatka crab.
    Biochemistry (Mosc). 2006 May;71(5):513-9
    PMID: 16732729

  22. Efimov VA, Birikh KR, Staroverov DB, Lukyanov SA, Tereshina MB, Zaraisky AG, Chakhmakhcheva OG.
    Hydroxyproline-based DNA mimics provide an efficient gene silencing in vitro and in vivo.
    Nucleic Acids Res. 2006 May 2;34(8):2247-57
    PMID: 16670431

  23. Gurskaya NG, Verkhusha VV, Shcheglov AS, Staroverov DB, Chepurnykh TV, Fradkov AF, Lukyanov S, Lukyanov KA.
    Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light.
    Nat Biotechnol. 2006 Apr;24(4):461-5
    PMID: 16550175

  24. Belousov VV, Fradkov AF, Lukyanov KA, Staroverov DB, Shakhbazov KS, Terskikh AV, Lukyanov S.
    Genetically encoded fluorescent indicator for intracellular hydrogen peroxide.
    Nat Methods. 2006 Apr;3(4):281-6
    PMID: 16554833

  25. Il'ina A, Lipkin A, Barsova E, Issaeva M, Leychenko E, Guzev K, Monastyrnaya M, Lukyanov S, Kozlovskaya E.
    Amino acid sequence of RTX-A's isoform actinoporin from the sea anemone, Radianthus macrodactylus.
    Toxicon. 2006 Apr;47(5):517-20
    PMID: 16530241

  26. Bulina ME, Chudakov DM, Britanova OV, Yanushevich YG, Staroverov DB, Chepurnykh TV, Merzlyak EM, Shkrob MA, Lukyanov S, Lukyanov KA.
    A genetically encoded photosensitizer.
    Nat Biotechnol. 2006 Jan;24(1):95-9
    PMID: 16369538

  27. Lukyanov KA, Chudakov DM, Fradkov AF, Labas YA, Matz MV and Lukyanov SA.
    Discovery and properties of GFP-like proteins from non-bioluminescent Anthozoa.
    In: Green fluorescent protein: properties and applications. Chalfie, M., Kain, S., (Eds). Willey-Liss, New York, 2005; 121-38

  28. Chudakov DM, Lukyanov S, Lukyanov KA.
    Fluorescent proteins as a toolkit for in vivo imaging.
    Trends Biotechnol. 2005 Dec;23(12):605-13
    PMID: 16269193

  29. Shkrob MA, Yanushevich YG, Chudakov DM, Gurskaya NG, Labas YA, Poponov SY, Mudrik NN, Lukyanov S, Lukyanov KA.
    Far-red fluorescent proteins evolved from a blue chromoprotein from Actinia equina.
    Biochem J. 2005 Dec 15;392(Pt 3):649-54
    PMID: 16164420

  30. Lukyanov KA, Chudakov DM, Lukyanov S, Verkhusha VV.
    Innovation: Photoactivatable fluorescent proteins.
    Nat Rev Mol Cell Biol. 2005 Nov;6(11):885-91
    PMID: 16167053

  31. Yampolsky IV, Remington SJ, Martynov VI, Potapov VK, Lukyanov S, Lukyanov KA.
    Synthesis and properties of the chromophore of the asFP595 chromoprotein from Anemonia sulcata.
    Biochemistry. 2005 Apr 19;44(15):5788-93
    PMID: 15823037

  32. Zhulidov PA, Bogdanova EA, Shcheglov AS, Shagina IA, Wagner LL, Khazpekov GL, Kozhemyako VV, Lukyanov SA, Shagin DA.
    A method for the preparation of normalized cDNA libraries enriched with full-length sequences.
    Bioorg Khim. 2005 Mar-Apr;31(2):186-94 (in Russian)
    PMID: 15889793

  33. Britanova O, Akopov S, Lukyanov S, Gruss P, Tarabykin V.
    Novel transcription factor Satb2 interacts with matrix attachment region DNA elements in a tissue-specific manner and demonstrates cell-type-dependent expression in the developing mouse CNS.
    Eur J Neurosci. 2005 Feb;21(3):658-68
    PMID: 15733084

  34. Yanushevich YG, Shagin DA, Fradkov AF, Shakhbazov KS, Barsova EV, Gurskaya NG, Labas YA, Matz MV, Lukyanov KA, Lukyanov SA.
    Spectral diversity among the members of the family of Green Fluorescent Protein in hydroid jellyfish (Cnidaria, Hydrozoa).
    Bioorg Khim. 2005 Jan-Feb;31(1):49-53 (in Russian)
    PMID: 15787213

  35. Verkhusha VV, Chudakov DM, Gurskaya NG, Lukyanov S, Lukyanov KA.
    Common pathway for the red chromophore formation in fluorescent proteins and chromoproteins.
    Chem Biol. 2004;11(6):845-54
    PMID: 15217617

  36. Rebrikov DV, Desai SM, Siebert PD, Lukyanov SA.
    Suppression subtractive hybridization.
    Methods Mol Biol. 2004;258:107-34
    PMID: 14970460

  37. Bogdanova EA, Barsova EV, Pounkova NI, Britanova OV, Shagin DA, Gurskaya NG, Usman NIu, Lukyanov SA.
    A family of genes of multidomain free lectins from a planarian: structure, expression, and use as markers for regeneration monitoring.
    Bioorg Khim. 2004 Nov-Dec;30(6):626-37 (in Russian)
    PMID: 15586815

  38. Chudakov DM, Verkhusha VV, Staroverov DB, Souslova EA, Lukyanov S, Lukyanov KA.
    Photoswitchable cyan fluorescent protein for protein tracking.
    Nat Biotechnol. 2004 Nov;22(11):1435-9
    PMID: 15502815

  39. Bulina ME, Lukyanov KA, Yampolsky IV, Chudakov DM, Staroverov DB, Shcheglov AS, Gurskaya NG, Lukyanov S.
    New class of blue animal pigments based on Frizzled and Kringle protein domains.
    J Biol Chem. 2004 Oct 15;279(42):43367-70
    PMID: 15297465

  40. Poliakov AS, Speer N, Britanova OV, Lukyanov SA, Tarabykin VS, Korochkin LI.
    Cloning and analysis of a new neurogene in the mouse.
    Genetika. 2004 Jun;40(6):853-7 (in Russian)
    PMID: 15341278

  41. Shagin DA, Barsova EV, Yanushevich YG, Fradkov AF, Lukyanov KA, Labas YA, Semenova TN, Ugalde JA, Meyers A, Nunez JM, Widder EA, Lukyanov SA, Matz MV.
    GFP-like proteins as ubiquitous Metazoan superfamily: evolution of functional features and structural complexity.
    Mol Biol Evol. 2004 May;21(5):841-50
    PMID: 14963095

  42. Baranova A, Ivanov D, Petrash N, Pestova A, Skoblov M, Kelmanson I, Shagin D, Nazarenko S, Geraymovych E, Litvin O, Tiunova A, Born TL, Usman N, Staroverov D, Lukyanov S, Panchin Y.
    The mammalian pannexin family is homologous to the invertebrate innexin gap junction proteins.
    Genomics. 2004 Apr;83(4):706-16
    PMID: 15028292

  43. Kozhemyako VB, Rebrikov DV, Lukyanov SA, Bogdanova EA, Marin A, Mazur AK, Kovalchuk SN, Agafonova EV, Sova VV, Elyakova LA, Rasskazov VA.
    Molecular cloning and characterization of an endo-1,3-beta-D-glucanase from the mollusk Spisula sachalinensis.
    Comp Biochem Physiol B Biochem Mol Biol. 2004 Feb;137(2):169-78
    PMID: 14990213

  44. Zhulidov PA, Bogdanova EA, Shcheglov AS, Vagner LL, Khaspekov GL, Kozhemyako VB, Matz MV, Meleshkevitch E, Moroz LL, Lukyanov SA, Shagin DA.
    Simple cDNA normalization using kamchatka crab duplex-specific nuclease.
    Nucleic Acids Res. 2004 Feb 18;32(3):e37
    PMID: 14973331

  45. Matz MV, Alieva NO, Chenchik A, Lukyanov S.
    Amplification of cDNA ends using PCR suppression effect and step-out PCR.
    Methods Mol Biol. 2003;221:41-9
    PMID: 12703732

  46. Baranova A, Hammarsund M, Ivanov D, Skoblov M, Sangfelt O, Corcoran M, Borodina T, Makeeva N, Pestova A, Tyazhelova T, Nazarenko S, Gorreta F, Alsheddi T, Schlauch K, Nikitin E, Kapanadze B, Shagin D, Poltaraus A, Ivanovich Vorobiev A, Zabarovsky E, Lukyanov S, Chandhoke V, Ibbotson R, Oscier D, Einhorn S, Grander D, Yankovsky N.
    Distinct organization of the candidate tumor suppressor gene RFP2 in human and mouse: multiple mRNA isoforms in both species- and human-specific antisense transcript RFP2OS.
    Gene. 2003 Dec 4;321:103-12
    PMID: 14636997

  47. Martynov VI, Maksimov BI, Martynova NY, Pakhomov AA, Gurskaya NG, Lukyanov SA.
    A purple-blue chromoprotein from Goniopora tenuidens belongs to the DsRed subfamily of GFP-like proteins.
    J Biol Chem. 2003 Nov 21;278(47):46288-92
    PMID: 12975373

  48. Polyakov AS, Britanova OV, Usman N, Pounkova NI, Lukyanov SA, Tarabykin VS, Korochkin LI.
    New neurogenes in mammals.
    Dokl Biol Sci. 2003 Sep-Oct;392:467-70 (in Russian)
    PMID: 14650889

  49. Yanushevich YG, Gurskaya NG, Staroverov DB, Lukyanov SA, Lukyanov KA.
    A natural fluorescent protein that changes its fluorescence during maturation.
    Bioorg Khim. 2003 Jul-Aug;29(4):356-60 (in Russian)
    PMID: 12947756

  50. Gurskaya NG, Fradkov AF, Pounkova NI, Staroverov DB, Bulina ME, Yanushevich YG, Labas YA, Lukyanov S, Lukyanov KA.
    A colourless green fluorescent protein homologue from the non-fluorescent hydromedusa Aequorea coerulescens and its fluorescent mutants.
    Biochem J. 2003 Jul 15;373(Pt 2):403-8
    PMID: 12693991

  51. Chudakov DM, Belousov VV, Zaraisky AG, Novoselov VV, Staroverov DB, Zorov DB, Lukyanov S, Lukyanov KA.
    Kindling fluorescent proteins for precise in vivo photolabeling.
    Nat Biotechnol. 2003 Feb;21(2):191-4
    PMID: 12524551

  52. Chudakov DM, Feofanov AV, Mudrik NN, Lukyanov S, Lukyanov KA.
    Chromophore environment provides clue to kindling fluorescent protein riddle.
    J Biol Chem. 2003 Feb 28;278(9):7215-9
    PMID: 12496281

  53. Kelmanson IV, Shagin DA, Usman N, Matz MV, Lukyanov SA, Panchin YV.
    Altering electrical connections in the nervous system of the pteropod mollusc Clione limacina by neuronal injections of gap junction mRNA.
    Eur J Neurosci. 2002 Dec;16(12):2475-6
    PMID: 12492443

  54. Shagin DA, Rebrikov DV, Kozhemyako VB, Altshuler IM, Shcheglov AS, Zhulidov PA, Bogdanova EA, Staroverov DB, Rasskazov VA, Lukyanov S.
    A novel method for SNP detection using a new duplex-specific nuclease from crab hepatopancreas.
    Genome Res. 2002 Dec;12(12):1935-42
    PMID: 12466298

  55. Rebrikov DV, Bogdanova EA, Bulina ME, Lukyanov SA.
    A new planarian extrachromosomal virus-like element revealed by subtraction hybridization.
    Mol Biol (Mosk). 2002 Nov-Dec;36(6):1002-11 (in Russian)
    PMID: 12500537

  56. Fradkov AF, Verkhusha VV, Staroverov DB, Bulina ME, Yanushevich YG, Martynov VI, Lukyanov S, Lukyanov KA.
    Far-red fluorescent tag for protein labelling.
    Biochem J. 2002 Nov 15;368(Pt 1):17-21
    PMID: 12350221

  57. Matz MV, Lukyanov KA, Lukyanov SA.
    Family of the green fluorescent protein: journey to the end of the rainbow.
    Bioessays. 2002 Oct;24(10):953-9
    PMID: 12325128

  58. Shagin DA, Barsova EV, Bogdanova E, Britanova OV, Gurskaya N, Lukyanov KA, Matz MV, Pounkova NI, Usman NY, Kopantzev EP, Salo E, Lukyanov SA.
    Identification and characterization of a new family of C-type lectin-like genes from planaria Girardia tigrina.
    Glycobiology. 2002 Aug;12(8):463-72
    PMID: 12145187

  59. Britanova O, Lukyanov S, Gruss P, Tarabykin V.
    The mouse Laf4 gene: exon/intron organization, cDNA sequence, alternative splicing, and expression during central nervous system development.
    Genomics. 2002 Jul;80(1):31-7
    PMID: 12079280

  60. Rebrikov DV, Bulina ME, Bogdanova EA, Wagner LL, Lukyanov SA.
    Complete genome sequence of a novel extrachromosomal virus-like element identified in planarian Girardia tigrina.
    BMC Genomics. 2002 Jun 13;3(1):15
    PMID: 12065025
    FULL TEXT

  61. Nikiforova NN, Velikodvorskaja TV, Kachko AV, Nikolaev LG, Monastyrskaya GS, Lukyanov SA, Konovalova SN, Protopopova EV, Svyatchenko VA, Kiselev NN, Loktev VB, Sverdlov ED.
    Induction of alternatively spliced spermidine/spermine N1-acetyltransferase mRNA in the human kidney cells infected by venezuelan equine encephalitis and tick-borne encephalitis viruses.
    Virology. 2002 Jun 5;297(2):163-71
    PMID: 12083816

  62. Barinova I, Zhexembekova M, Barsova E, Lukyanov S, Heberle-Bors E, Touraev A.
    Antirrhinum majus microspore maturation and transient transformation in vitro.
    J Exp Bot. 2002 May;53(371):1119-29
    PMID: 11971923

  63. Labas YA, Gurskaya NG, Yanushevich YG, Fradkov AF, Lukyanov KA, Lukyanov SA, Matz MV.
    Diversity and evolution of the green fluorescent protein family.
    Proc Natl Acad Sci U S A. 2002 Apr 2;99(7):4256-61
    PMID: 11929996

  64. Yanushevich YG, Staroverov DB, Savitsky AP, Fradkov AF, Gurskaya NG, Bulina ME, Lukyanov KA, Lukyanov SA.
    A strategy for the generation of non-aggregating mutants of Anthozoa fluorescent proteins.
    FEBS Lett. 2002 Jan 30;511(1-3):11-4
    PMID: 11821040

  65. Gurskaya NG, Savitsky AP, Yanushevich YG, Lukyanov SA, Lukyanov KA.
    Color transitions in coral's fluorescent proteins by site-directed mutagenesis.
    BMC Biochem. 2001;2:6
    PMID: 11459517
    FULL TEXT

  66. Gurskaya NG, Fradkov AF, Terskikh A, Matz MV, Labas YA, Martynov VI, Yanushevich YG, Lukyanov KA, Lukyanov SA.
    GFP-like chromoproteins as a source of far-red fluorescent proteins.
    FEBS Lett. 2001 Oct 19;507(1):16-20
    PMID: 11682051

  67. Martynov VI, Savitsky AP, Martynova NY, Savitsky PA, Lukyanov KA, Lukyanov SA.
    Alternative cyclization in GFP-like proteins family. The formation and structure of the chromophore of a purple chromoprotein from Anemonia sulcata.
    J Biol Chem. 2001 Jun 15;276(24):21012-6
    PMID: 11259412

  68. Terskikh A, Fradkov A, Ermakova G, Zaraisky A, Tan P, Kajava AV, Zhao X, Lukyanov S, Matz M, Kim S, Weissman I, Siebert P.
    "Fluorescent timer": protein that changes color with time.
    Science. 2000 Nov 24;290(5496):1585-8
    PMID: 11090358

  69. Rebrikov DV, Britanova OV, Gurskaya NG, Lukyanov KA, Tarabykin VS, Lukyanov SA.
    Mirror orientation selection (MOS): a method for eliminating false positive clones from libraries generated by suppression subtractive hybridization.
    Nucleic Acids Res. 2000 Oct 15;28(20):E90
    PMID: 11024192

  70. Lukyanov KA, Fradkov AF, Gurskaya NG, Matz MV, Labas YA, Savitsky AP, Markelov ML, Zaraisky AG, Zhao X, Fang Y, Tan W, Lukyanov SA.
    Natural animal coloration can be determined by a nonfluorescent green fluorescent protein homolog.
    J Biol Chem. 2000 Aug 25;275(34):25879-82
    PMID: 10852900

  71. Fradkov AF, Chen Y, Ding L, Barsova EV, Matz MV, Lukyanov SA.
    Novel fluorescent protein from Discosoma coral and its mutants possesses a unique far-red fluorescence.
    FEBS Lett. 2000 Aug 18;479(3):127-30
    PMID: 10981720

  72. Panchin Y, Kelmanson I, Matz M, Lukyanov K, Usman N, Lukyanov S.
    A ubiquitous family of putative gap junction molecules.
    Curr Biol. 2000 Jun 29;10(13):R473-4
    PMID: 10898987

  73. Tarabykin V, Britanova O, Fradkov A, Voss A, Katz LS, Lukyanov S, Gruss P.
    Expression of PTTG and prc1 genes during telencephalic neurogenesis.
    Mech Dev. 2000 Apr;92(2):301-4
    PMID: 10727870

  74. Zavalova LL, Baskova IP, Lukyanov SA, Sass AV, Snezhkov EV, Akopov SB, Artamonova II, Archipova VS, Nesmeyanov VA, Kozlov DG, Benevolensky SV, Kiseleva VI, Poverenny AM, Sverdlov ED.
    Destabilase from the medicinal leech is a representative of a novel family of lysozymes.
    Biochim Biophys Acta. 2000 Mar 16;1478(1):69-77
    PMID: 10719176

  75. Diatchenko L, Lukyanov S, Lau YF, Siebert PD.
    Suppression subtractive hybridization: a versatile method for identifying differentially expressed genes.
    Methods Enzymol. 1999;303:349-80
    PMID: 10349654

  76. Bogush ML, Velikodvorskaya TV, Lebedev YB, Nikolaev LG, Lukyanov SA, Fradkov AF, Pliyev BK, Boichenko MN, Usatova GN, Vorobiev AA, Andersen GL, Sverdlov ED.
    Identification and localization of differences between Escherichia coli and Salmonella typhimurium genomes by suppressive subtractive hybridization.
    Mol Gen Genet. 1999 Dec;262(4-5):721-9
    PMID: 10628854

  77. Matz MV, Fradkov AF, Labas YA, Savitsky AP, Zaraisky AG, Markelov ML, Lukyanov SA.
    Fluorescent proteins from nonbioluminescent Anthozoa species.
    Nat Biotechnol. 1999 Oct;17(10):969-73
    PMID: 10504696

  78. Danilevich VN, Lukyanov SA, Grishin EV.
    Cloning and structure of gene encoded alpha-latrocrustoxin from the Black widow spider venom.
    Bioorg Khim. 1999 Jul;25(7):537-47 (in Russian)
    PMID: 10563207

  79. Broude NE, Storm N, Malpel S, Graber JH, Lukyanov S, Sverdlov E, Smith CL.
    PCR based targeted genomic and cDNA differential display.
    Genet Anal. 1999 Apr;15(2):51-63
    PMID: 10191986

  80. Lukyanov KA, Gurskaya NG, Bogdanova EA, Lukyanov SA.
    Selective suppression of polymerase chain reaction.
    Bioorg Khim. 1999 Mar;25(3):163-70 (in Russian)
    PMID: 10382038
    FULL TEXT

  81. Matz M, Shagin D, Bogdanova E, Britanova O, Lukyanov S, Diatchenko L, Chenchik A.
    Amplification of cDNA ends based on template-switching effect and step-out PCR.
    Nucleic Acids Res. 1999 Mar 15;27(6):1558-60
    PMID: 10037822

  82. Lavrentieva I, Broude NE, Lebedev Y, Gottesman II, Lukyanov SA, Smith CL, Sverdlov ED.
    High polymorphism level of genomic sequences flanking insertion sites of human endogenous retroviral long terminal repeats.
    FEBS Lett. 1999 Jan 29;443(3):341-7
    PMID: 10025960

  83. Matz MV, Shagin DA, Usman NIu, Bogdanova EA, Fradkov AF, Soboleva TA, Lukyanov SA.
    Cloning of region-specific genetic markers of planaria using a new method--ordered differential display.
    Bioorg Khim. 1998 Dec;24(12):910-5 (in Russian)
    PMID: 10079949

  84. Matz MV, Lukyanov SA.
    Different strategies of differential display: areas of application.
    Nucleic Acids Res. 1998 Dec 15;26(24):5537-43
    PMID: 9837980

  85. Akopyants NS, Fradkov A, Diatchenko L, Hill JE, Siebert PD, Lukyanov SA, Sverdlov ED, Berg DE.
    PCR-based subtractive hybridization and differences in gene content among strains of Helicobacter pylori.
    Proc Natl Acad Sci U S A. 1998 Oct 27;95(22):13108-13
    PMID: 9789049

  86. Fradkov AF, Lukyanov KA, Matz MV, Diatchenko LB, Siebert PD, Lukyanov SA.
    Sequence-independent method for in vitro generation of nested deletions for sequencing large DNA fragments.
    Anal Biochem. 1998 Apr 10;258(1):138-41
    PMID: 9527860

  87. Bogdanova E, Matz M, Tarabykin V, Usman N, Shagin D, Zaraisky A, Lukyanov S.
    Inductive interactions regulating body patterning in planarian, revealed by analysis of expression of novel gene scarf.
    Dev Biol. 1998 Feb 15;194(2):172-81
    PMID: 9501028

  88. Vasiliev OL, Lukyanov SA, Belyavsky AV, Kazanskaya OV, Zaraisky AG.
    A novel marker of early epidermal differentiation: cDNA subtractive cloning starting on a single explant of Xenopus laevis gastrula epidermis.
    Int J Dev Biol. 1997 Dec;41(6):877-82
    PMID: 9449464

  89. Lukyanov KA, Lukyanov SA.
    In vitro cloning of DNA fragments by one polymerase chain reaction.
    Bioorg Khim. 1997 Nov;23(11):882-7 (in Russian)
    PMID: 9518428

  90. Kazanskaya OV, Severtzova EA, Barth KA, Ermakova GV, Lukyanov SA, Benyumov AO, Pannese M, Boncinelli E, Wilson SW, Zaraisky AG.
    Anf: a novel class of vertebrate homeobox genes expressed at the anterior end of the main embryonic axis.
    Gene. 1997 Oct 24;200(1-2):25-34
    PMID: 9373136

  91. Markitantova IuV, Lukyanov KA, Kazanskaia OV, Mitashov VI, Lukyanov SA.
    An analysis of the expression of the genes containing the LeR-1 and VeR-1 sequences in the embryogenesis, regeneration and in the intact tissues of newts.
    Ontogenez. 1997 Jul-Aug;28(4):262-70 (in Russian)
    PMID: 9304090

  92. Matz M, Usman N, Shagin D, Bogdanova E, Lukyanov S.
    Ordered differential display: a simple method for systematic comparison of gene expression profiles.
    Nucleic Acids Res. 1997 Jun 15;25(12):2541-2
    PMID: 9171114

  93. Bogdanova EA, Matz MV, Tarabykin VS, Usman NIu, Lukyanov SA.
    Differential gene expression during the reparative regeneration of differing polarities in planarians.
    Ontogenez. 1997 Mar-Apr;28(2):132-7 (in Russian)
    PMID: 9173352

  94. Lukyanov K, Diatchenko L, Chenchik A, Nanisetti A, Siebert P, Usman N, Matz M, Lukyanov S.
    Construction of cDNA libraries from small amounts of total RNA using the suppression PCR effect.
    Biochem Biophys Res Commun. 1997 Jan 13;230(2):285-8
    PMID: 9016767

  95. Ermolaeva OD, Lukyanov SA, Sverdlov ED.
    The mathematical model of subtractive hybridization and its practical application.
    Proc Int Conf Intell Syst Mol Biol. 1996;4:52-8
    PMID: 8877504

  96. Vasiliev OL, Lukyanov SA, Belyavsky AV, Kazanskaya OV, Zaraisky AG.
    A novel method of subtraction cDNA hybridization allowing cloning of genes showing differential expression in cell micropopulations.
    Bioorg Khim. 1996 Dec;22(12):894-9 (in Russian)
    PMID: 9054339

  97. Zavalova L, Lukyanov S, Baskova I, Snezhkov E, Akopov S, Berezhnoy S, Bogdanova E, Barsova E, Sverdlov ED.
    Genes from the medicinal leech (Hirudo medicinalis) coding for unusual enzymes that specifically cleave endo-epsilon (gamma-Glu)-Lys isopeptide bonds and help to dissolve blood clots.
    Mol Gen Genet. 1996 Nov 27;253(1-2):20-5
    PMID: 9003282

  98. Lukyanov KA, Gurskaya NG, Matz MV, Khaspekov GL, Diatchenko LB, Chenchik AA, Il'evich-Stuchkov SG, Lukyanov SA.
    A method for obtaining the normalized cDNA libraries based on the effect of suppression of polymerase chain reaction.
    Bioorg Khim. 1996 Sep;22(9):686-90 (in Russian)
    PMID: 8999786

  99. Chenchik A, Diatchenko L, Moqadam F, Tarabykin V, Lukyanov S, Siebert PD.
    Full-length cDNA cloning and determination of mRNA 5' and 3' ends by amplification of adaptor-ligated cDNA.
    Biotechniques. 1996 Sep;21(3):526-34
    PMID: 8879595

  100. Gurskaya NG, Diatchenko L, Chenchik A, Siebert PD, Khaspekov GL, Lukyanov KA, Wagner LL, Ermolaeva OD, Lukyanov SA, Sverdlov ED.
    Equalizing cDNA subtraction based on selective suppression of polymerase chain reaction: cloning of Jurkat cell transcripts induced by phytohemaglutinin and phorbol 12-myristate 13-acetate.
    Anal Biochem. 1996 Aug 15;240(1):90-7
    PMID: 8811883

  101. Fradkov A, Berezhnoy S, Barsova E, Zavalova L, Lukyanov S, Baskova I, Sverdlov ED.
    Enzyme from the medicinal leech (Hirudo medicinalis) that specifically splits endo-epsilon(-gamma-Glu)-Lys isopeptide bonds: cDNA cloning and protein primary structure.
    FEBS Lett. 1996 Jul 22;390(2):145-8
    PMID: 8706846

  102. Gurskaya NG, Shagin DA, Lukyanov KA, Wagner LL, Shtutman MS, Musatkina EA, Moinova EV, Tatosian AG, Lukyanov SA, Sverdlov ED.
    Cloning cDNA for the ha-SDGF gene from a Syrian hamster cell line with increased metastatic potential using subtractive hybridization.
    Bioorg Khim. 1996 Jun;22(6):425-31 (in Russian)
    PMID: 8975671

  103. Diatchenko L, Lau YF, Campbell AP, Chenchik A, Moqadam F, Huang B, Lukyanov S, Lukyanov K, Gurskaya N, Sverdlov ED, Siebert PD.
    Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries.
    Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):6025-30
    PMID: 8650213

  104. Lukyanov KA, Matz MV, Bogdanova EA, Gurskaya NG, Lukyanov SA.
    Molecule by molecule PCR amplification of complex DNA mixtures for direct sequencing: an approach to in vitro cloning.
    Nucleic Acids Res. 1996 Jun 1;24(11):2194-5
    PMID: 8668555

  105. Lukyanov KA, Gurskaya NG, Kopantsev EP, Lukyanov SA.
    Selective amplification of evolutionally conserved expressed sequences.
    Bioorg Khim. 1996 Jan;22(1):48-53 (in Russian)
    PMID: 8651955

  106. Zaraisky AG, Ecochard V, Kazanskaya OV, Lukyanov SA, Fesenko IV, Duprat AM.
    The homeobox-containing gene XANF-1 may control development of the Spemann organizer.
    Development. 1995 Nov;121(11):3839-47
    PMID: 8582293

  107. Lukyanov KA, Launer GA, Tarabykin VS, Zaraisky AG, Lukyanov SA.
    Inverted terminal repeats permit the average length of amplified DNA fragments to be regulated during preparation of cDNA libraries by polymerase chain reaction.
    Anal Biochem. 1995 Aug 10;229(2):198-202
    PMID: 7485972

  108. Tarabykin VS, Lukyanov KA, Potapov VK, Lukyanov SA.
    Detection of planarian Antennapedia-like homeobox genes expressed during regeneration.
    Gene. 1995 Jun 9;158(2):197-202
    PMID: 7607541

  109. Mitashov VI, Lukyanov SA, Kazanskaia OV, Markitantova IuV, Dolgilevich SM, Snegovaia IIu, Znoĭko SL, Mikaélian AS.
    Molecular-biological approaches to studying gene expression during regeneration.
    Izv Akad Nauk Ser Biol. 1995 May-Jun;(3):276-80 (in Russian)
    PMID: 7670351

  110. Kazanskaia OV, Markitantova IuV, Snegovaia IIu, Dolgilevich SM, Tarabykin VS, Zaraisky AG, Lukyanov SA, Znoĭko SL, Mikaélian AS, Mitashov VI.
    Identification of new genes and analysis of their expression during lens and retinal regeneration in adult newts.
    Izv Akad Nauk Ser Biol. 1995 May-Jun;(3):271-5 (in Russian)
    PMID: 7670350

  111. Siebert PD, Chenchik A, Kellogg DE, Lukyanov KA, Lukyanov SA.
    An improved PCR method for walking in uncloned genomic DNA.
    Nucleic Acids Res. 1995 Mar 25;23(6):1087-8
    PMID: 7731798

  112. Lukyanov S, Gurskaya NG, Lukyanov K, Tarabykin VS, Sverdlov ED.
    Highly efficient subtractive hybridisation of cDNA.
    Bioorg Khim. 1994;20:701-4 (in Russian)
    PMID: 7945464

  113. Launer GA, Lukyanov KA, Tarabykin VS, Lukyanov SA.
    Simple method for cDNA amplification starting from small amount of total RNA.
    Mol Gen Mikrobiol Virusol. 1994 Nov-Dec;(6):38-41 (in Russian)
    PMID: 7537858

  114. Lukyanov K, Tarabykin VS, Potapov VK, Bekman P, Lukyanov SA.
    The cloning of fragments of homeobox genes expressed during planarian regeneration.
    Ontogenez. 1994 Nov-Dec;25(6):28-32 (in Russian)
    PMID: 7777253

  115. Zaraisky AG, Lukyanov SA, Vasiliev OL, Smirnov YV, Belyavsky AV, Kazanskaya OV.
    A novel homeobox gene expressed in the anterior neural plate of the Xenopus embryo.
    Dev Biol. 1992 Aug;152(2):373-82
    PMID: 1353734

  116. Lukyanov SA, Zaraisky AG.
    The space-time distribution of the mRNA of the nuclear proteins c-myc and P-53 in the development of the clawed toad studied by hybridization in situ.
    Ontogenez. 1991 Jan-Feb;22(1):47-52 (in Russian)
    PMID: 1857586

  117. Lukyanov SA, Zaraisky AG.
    Methodological approaches to detecting mRNA in histological sections.
    Ontogenez. 1990 Sep-Oct;21(5):455-65 (in Russian)
    PMID: 1704117

  118. Zaraisky AG, Lukyanov SA, Ermakova GV.
    The spatio-temporal distribution of single-stranded breaks in nuclear DNA in sections of clawed toad embryos during gastrulation and neurulation.
    Ontogenez. 1989 Sep-Oct;20(5):471-7 (in Russian)
    PMID: 2587008

  119. Zaraisky AG, Lukyanov SA, Laman AG.
    The spatial-temporal distribution of the mRNA of the Na+-K+-ATPase alpha-subunit in the early development of the clawed toad studied by hybridization in situ.
    Ontogenez. 1989 Mar-Apr;20(2):128-34 (in Russian)
    PMID: 2544838