Shemyakin & Ovchinikov Institute of Bioorganic Chemistry
Laboratory of Molecular Technologies
| Home | People | Research | Publications | Protocols | Collaborations |

Selective suppression of the polymerase chain reaction and methods based upon it

  1. Buzdin A, Lukyanov S.
    Stem-loop oligonucleotides as hybridization probes and their practical use in molecular biology and biomedicine.
    In: Nucleic Acids Hybridization: Modern Applications. Buzdin, Anton; Lukyanov, Sergey (Eds.) Hardcover, 2007; 85-96 ISBN: 978-1-4020-6039-7

  2. Lukyanov S, Rebrikov D, Buzdin A.
    Suppression subtractive hybridization
    In: Nucleic Acids Hybridization: Modern Applications. Buzdin, Anton; Lukyanov, Sergey (Eds.) Hardcover, 2007; 53-84 ISBN: 978-1-4020-6039-7

  3. Lukyanov S, Lukyanov K, Gurskaya N, Bogdanova E, Buzdin A.
    Selective suppression of polymerase chain reaction and its most popular applications.
    In: Nucleic Acids Hybridization: Modern Applications. Buzdin, Anton; Lukyanov, Sergey (Eds.) Hardcover, 2007; 29-51 ISBN: 978-1-4020-6039-7

  4. Hosp J, Tashpulatov A, Roessner U, Barsova E, Katholnigg H, Steinborn R, Melikant B, Lukyanov S, Heberle-Bors E, Touraev A.
    Transcriptional and metabolic profiles of stress-induced, embryogenic tobacco microspores.
    Plant Mol Biol. 2007 Jan;63(1):137-49
    PMID: 17016740

  5. Rebrikov DV, Desai SM, Siebert PD, Lukyanov SA.
    Suppression subtractive hybridization.
    Methods Mol Biol. 2004;258:107-34
    PMID: 14970460

  6. Rebrikov DV, Kogan YN, Diatchenko L.
    SSH subtractive cloning: comprehensive survey of genome differences in prokaryotes.
    In: Applications of Genomics and Proteomics for Analysis of Bacterial Biological Warfare Agents. V.G. DelVecchio and V. Krcmery (eds.). IOS Press, Amsterdam , Netherlands. 2003;352:95-105

  7. Rebrikov DV.
    Identification of differential genes by suppression subtractive hybridization.
    In: PCR Primer: A Laboratory Manual Second Edition Dieffenbach C. W. Dveksler G.S. (eds) Cold Spring Harbor Laboratory Press, New York, 2003; 297-325

  8. Matz MV, Alieva NO, Chenchik A, Lukyanov S.
    Amplification of cDNA ends using PCR suppression effect and step-out PCR.
    Methods Mol Biol. 2003;221:41-9
    PMID: 12703732

  9. Rebrikov D, Desai S, Kogan YN, Thornton AM, Diatchenko L.
    Subtractive cloning: new genes for studying inflammatory disorders.
    Ann Periodontol. 2002 Dec;7(1):17-28
    PMID: 16013213

  10. Rebrikov DV, Bogdanova EA, Bulina ME, Lukyanov SA.
    A new planarian extrachromosomal virus-like element revealed by subtraction hybridization.
    Mol Biol (Mosk). 2002 Nov-Dec;36(6):1002-11 (in Russian)
    PMID: 12500537

  11. Rebrikov DV, Bulina ME, Bogdanova EA, Wagner LL, Lukyanov SA.
    Complete genome sequence of a novel extrachromosomal virus-like element identified in planarian Girardia tigrina.
    BMC Genomics. 2002 Jun 13;3(1):15
    PMID: 12065025

  12. Rebrikov DV, Britanova OV, Gurskaya NG, Lukyanov KA, Tarabykin VS, Lukyanov SA.
    Mirror orientation selection (MOS): a method for eliminating false positive clones from libraries generated by suppression subtractive hybridization.
    Nucleic Acids Res. 2000 Oct 15;28(20):E90
    PMID: 11024192

  13. Bogush ML, Velikodvorskaya TV, Lebedev YB, Nikolaev LG, Lukyanov SA, Fradkov AF, Pliyev BK, Boichenko MN, Usatova GN, Vorobiev AA, Andersen GL, Sverdlov ED.
    Identification and localization of differences between Escherichia coli and Salmonella typhimurium genomes by suppressive subtractive hybridization.
    Mol Gen Genet. 1999 Dec;262(4-5):721-9
    PMID: 10628854

  14. Shagin DA, Lukyanov KA, Wagner LL, Matz MV.
    Regulation of average length of complex PCR product.
    Nucleic Acids Res. 1999 Sep 15;27(18):e23
    PMID: 10471753

  15. Broude NE, Storm N, Malpel S, Graber JH, Lukyanov S, Sverdlov E, Smith CL.
    PCR based targeted genomic and cDNA differential display.
    Genet Anal. 1999 Apr;15(2):51-63
    PMID: 10191986

  16. Lukyanov KA, Gurskaya NG, Bogdanova EA, Lukyanov SA.
    Selective suppression of polymerase chain reaction.
    Bioorg Khim. 1999 Mar;25(3):163-70 (in Russian)
    PMID: 10382038

  17. Matz M, Shagin D, Bogdanova E, Britanova O, Lukyanov S, Diatchenko L, Chenchik A.
    Amplification of cDNA ends based on template-switching effect and step-out PCR.
    Nucleic Acids Res. 1999 Mar 15;27(6):1558-60
    PMID: 10037822

  18. Lukyanov K.
    In vitro cloning: a method for PCR amplification of individual unknown DNA fragments suitable for direct sequencing.
    In: Gene Cloning and Analysis by RT-PCR. Ed. by P. Siebert and J. Larrick, BioTechniques Books, Natick, MA, 1998; 329-45

  19. Chen SS, Chenchik A, Lukyanov K, Siebert PD.
    Improved technique for walking in uncloned genomic DNA.
    In: Gene Cloning and Analysis by RT-PCR. Ed. by P. Siebert and J. Larrick, BioTechniques Books, Natick, MA, 1998; 289-304

  20. Matz MV, Shagin DA, Usman NIu, Bogdanova EA, Fradkov AF, Soboleva TA, Lukyanov SA.
    Cloning of region-specific genetic markers of planaria using a new method--ordered differential display.
    Bioorg Khim. 1998 Dec;24(12):910-5 (in Russian)
    PMID: 10079949

  21. Matz MV, Lukyanov SA.
    Different strategies of differential display: areas of application.
    Nucleic Acids Res. 1998 Dec 15;26(24):5537-43
    PMID: 9837980

  22. Akopyants NS, Fradkov A, Diatchenko L, Hill JE, Siebert PD, Lukyanov SA, Sverdlov ED, Berg DE.
    PCR-based subtractive hybridization and differences in gene content among strains of Helicobacter pylori.
    Proc Natl Acad Sci U S A. 1998 Oct 27;95(22):13108-13
    PMID: 9789049

  23. Fradkov AF, Lukyanov KA, Matz MV, Diatchenko LB, Siebert PD, Lukyanov SA.
    Sequence-independent method for in vitro generation of nested deletions for sequencing large DNA fragments.
    Anal Biochem. 1998 Apr 10;258(1):138-41
    PMID: 9527860

  24. Lukyanov KA, Lukyanov SA.
    In vitro cloning of DNA fragments by one polymerase chain reaction.
    Bioorg Khim. 1997 Nov;23(11):882-7 (in Russian)
    PMID: 9518428

  25. Markitantova IuV, Lukyanov KA, Kazanskaia OV, Mitashov VI, Lukyanov SA.
    An analysis of the expression of the genes containing the LeR-1 and VeR-1 sequences in the embryogenesis, regeneration and in the intact tissues of newts.
    Ontogenez. 1997 Jul-Aug;28(4):262-70 (in Russian)
    PMID: 9304090

  26. Matz M, Usman N, Shagin D, Bogdanova E, Lukyanov S.
    Ordered differential display: a simple method for systematic comparison of gene expression profiles.
    Nucleic Acids Res. 1997 Jun 15;25(12):2541-2
    PMID: 9171114

  27. Lukyanov K, Diatchenko L, Chenchik A, Nanisetti A, Siebert P, Usman N, Matz M, Lukyanov S.
    Construction of cDNA libraries from small amounts of total RNA using the suppression PCR effect.
    Biochem Biophys Res Commun. 1997 Jan 13;230(2):285-8
    PMID: 9016767

  28. Ermolaeva OD, Lukyanov SA, Sverdlov ED.
    The mathematical model of subtractive hybridization and its practical application.
    Proc Int Conf Intell Syst Mol Biol. 1996;4:52-8
    PMID: 8877504

  29. Lukyanov KA, Gurskaya NG, Matz MV, Khaspekov GL, Diatchenko LB, Chenchik AA, Il'evich-Stuchkov SG, Lukyanov SA.
    A method for obtaining the normalized cDNA libraries based on the effect of suppression of polymerase chain reaction.
    Bioorg Khim. 1996 Sep;22(9):686-90 (in Russian)
    PMID: 8999786

  30. Chenchik A, Diatchenko L, Moqadam F, Tarabykin V, Lukyanov S, Siebert PD.
    Full-length cDNA cloning and determination of mRNA 5' and 3' ends by amplification of adaptor-ligated cDNA.
    Biotechniques. 1996 Sep;21(3):526-34
    PMID: 8879595

  31. Gurskaya NG, Diatchenko L, Chenchik A, Siebert PD, Khaspekov GL, Lukyanov KA, Wagner LL, Ermolaeva OD, Lukyanov SA, Sverdlov ED.
    Equalizing cDNA subtraction based on selective suppression of polymerase chain reaction: cloning of Jurkat cell transcripts induced by phytohemaglutinin and phorbol 12-myristate 13-acetate.
    Anal Biochem. 1996 Aug 15;240(1):90-7
    PMID: 8811883

  32. Gurskaya NG, Shagin DA, Lukyanov KA, Wagner LL, Shtutman MS, Musatkina EA, Moinova EV, Tatosian AG, Lukyanov SA, Sverdlov ED.
    Cloning cDNA for the ha-SDGF gene from a Syrian hamster cell line with increased metastatic potential using subtractive hybridization.
    Bioorg Khim. 1996 Jun;22(6):425-31 (in Russian)
    PMID: 8975671

  33. Diatchenko L, Lau YF, Campbell AP, Chenchik A, Moqadam F, Huang B, Lukyanov S, Lukyanov K, Gurskaya N, Sverdlov ED, Siebert PD.
    Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries.
    Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):6025-30
    PMID: 8650213

  34. Lukyanov KA, Matz MV, Bogdanova EA, Gurskaya NG, Lukyanov SA.
    Molecule by molecule PCR amplification of complex DNA mixtures for direct sequencing: an approach to in vitro cloning.
    Nucleic Acids Res. 1996 Jun 1;24(11):2194-5
    PMID: 8668555

  35. Lukyanov KA, Gurskaya NG, Kopantsev EP, Lukyanov SA.
    Selective amplification of evolutionally conserved expressed sequences.
    Bioorg Khim. 1996 Jan;22(1):48-53 (in Russian)
    PMID: 8651955

  36. Lukyanov KA, Launer GA, Tarabykin VS, Zaraisky AG, Lukyanov SA.
    Inverted terminal repeats permit the average length of amplified DNA fragments to be regulated during preparation of cDNA libraries by polymerase chain reaction.
    Anal Biochem. 1995 Aug 10;229(2):198-202
    PMID: 7485972

  37. Siebert PD, Chenchik A, Kellogg DE, Lukyanov KA, Lukyanov SA.
    An improved PCR method for walking in uncloned genomic DNA.
    Nucleic Acids Res. 1995 Mar 25;23(6):1087-8
    PMID: 7731798

  38. Lukyanov S, Gurskaya NG, Lukyanov K, Tarabykin VS, Sverdlov ED.
    Highly efficient subtractive hybridisation of cDNA.
    Bioorg Khim. 1994;20:701-4 (in Russian)
    PMID: 7945464

  39. Launer GA, Lukyanov KA, Tarabykin VS, Lukyanov SA.
    Simple method for cDNA amplification starting from small amount of total RNA.
    Mol Gen Mikrobiol Virusol. 1994 Nov-Dec;(6):38-41 (in Russian)
    PMID: 7537858