Bioorg Khim, 2000, 26(8):587-592

The solid-state catalytic isotope exchange of hydrogen in α-conotoxin G1 by the tritium spillover

Это дубликат статьи: «The solid-state catalytic isotope exchange of hydrogen in α-conotoxin G1 by the tritium spillover»

Tritium-labeled α-conotoxin G1 with a molar radioactivity of 35 Ci/mmol and full biological activity (according to the binding to nicotinic acetylcholine receptor) was obtained by the high-temperature solid-state catalytic isotope exchange (HSCIE). The tritium distribution in the molecule of α-conotoxin G1 was revealed by 3H NMR spectroscopy. Tritium was found in all amino acid residues except for the Asn4-Pro5-Ala6 fragment. The data on the comparative reactivity of C-H bonds, the ab initio quantum-chemical calculation of the hydrogen exchange reaction, and the information on the spatial structures of α-conotoxin G1 in solution and in crystal state allowed us to establish that the reactivity of H atoms may be increased by their interaction with the electron donor O and N atoms at the transition state of the HSCIE reaction. A decrease in the rate of the HSCIE reaction could be caused by both a poor spatial accessibility of C-H bonds and a limited mobility of the peptide fragment containing these bonds.

Zolotarev YA, Bocharov EV, Dadayan AK, Kasheverov IE, Zhmak MN, Maslennikov IV, Borisov YA, Arseniev AS, Myasoedov NF, Tsetlin VI

IBCH: 1971
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