Biochem J, 2009, 418(3):567-574

Far-red fluorescent tags for protein imaging in living tissues

A vast colour palette of monomeric fluorescent proteins has been developed to investigate protein localization, motility and interactions. However, low brightness has remained a problem in far-red variants, which hampers multicolour labelling and whole-body imaging techniques. In the present paper, we report mKate2, a monomeric far-red fluorescent protein that is almost 3-fold brighter than the previously reported mKate and is 10-fold brighter than mPlum. The high-brightness, far-red emission spectrum, excellent pH resistance and photostability, coupled with low toxicity demonstrated in transgenic Xenopus laevis embryos, make mKate2 a superior fluorescent tag for imaging in living tissues. We also report tdKatushka2, a tandem far-red tag that performs well in fusions, provides 4-fold brighter near-IR fluorescence compared with mRaspberry or mCherry, and is 20-fold brighter than mPlum. Together, monomeric mKate2 and pseudomonomeric tdKatushka2 represent the next generation of extrabright far-red fluorescent probes offering novel possibilities for fluorescent imaging of proteins in living cells and animals. © The Authors Journal compilation. © 2009 Biochemical Society.

Shcherbo D, Murphy CS, Ermakova GV, Solovieva EA, Chepurnykh TV, Shcheglov AS, Verkhusha VV, Pletnev VZ, Hazelwood KL, Roche PM, Lukyanov S, Zaraisky AG, Davidson MW, Chudakov DM

IBCH: 454
Ссылка на статью в журнале: http://biochemj.org/lookup/doi/10.1042/BJ20081949
Кол-во цитирований на 10.2023: 403
Информация пока не проверена модераторами