Russ. J. Bioorganic Chem., 2013, 39(1):72-76

Affinity capture of specific DNA fragments with short synthetic sequences

The ability of short peptide nucleic acid (PNA) oligomers and oligonucleotides containing modified residues of 5-methylcitidine, 2-aminoadenosine, and 5-propynyl-2′-deoxyuridine (strong binding oligonucleotides, SBO) to affinity capture the target double-stranded DNA fragment from mixture by means of the end invasion was compared. Both types of probes were highly effective at the conditions used. The SBO-based probes may represent a handy and easily prepared alternative to PNA for selection of target DNA fragments in mixtures. © 2013 Pleiades Publishing, Ltd.

Mikhailov VS, Potapov VK, Amirkhanov RN, Amirkhanov NV, Bulanenkova SS, Akopov SB, Zarytova VF, Nikolaev LG, Sverdlov ED

IBCH: 4739
Ссылка на статью в журнале: http://link.springer.com/10.1134/S106816201301007X
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