EMBO J, 2004, 23(21):4232-4242

Members of the SAGA and Mediator complexes are partners of the transcription elongation factor TFIIS

TFIIS, an elongation factor encoded by DST1 in Saccharomyces cerevisiae, stimulates transcript cleavage in arrested RNA polymerase II. Two components of the RNA polymerase II machinery, Med13 (Srb9) and Spt8, were isolated as two-hybrid partners of the conserved TFIIS N-terminal domain. They belong to the Cdk8 module of the Mediator and to a subform of the SAGA co-activator, respectively. Co-immunoprecipitation experiments showed that TFIIS can bind the Cdk8 module and SAGA in cell-free extracts. spt8Δ and dst1Δ mutants were sensitive to nucleotide-depleting drugs and epistatic to null mutants of the RNA polymerase II subunit Rpb9, suggesting that their elongation defects are mediated by Rpb9. rpb9Δ, spt8Δ and dst1Δ were lethal in cells lacking the Rpb4 subunit. The TFIIS N-terminal domain is also strictly required for viability in rpb4Δ, although it is not needed for binding to RNA polymerase II or for transcript cleavage. It is proposed that TFIIS and the Spt8-containing form of SAGA co-operate to rescue RNA polymerase II from unproductive elongation complexes, and that the Cdk8 module temporarily blocks transcription during transcript cleavage.

Wery M, Shematorova E, Van Driessche B, Vandenhaute J, Thuriaux P, Van Mullem V

IBCH: 5128
Ссылка на статью в журнале: http://emboj.embopress.org/cgi/doi/10.1038/sj.emboj.7600326
Кол-во цитирований на 12.2023: 58
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