Bioorg Khim, 2001, 27(5):358

Antibodies against synthetic fragments of the prion protein for diagnostics of bovine spongiform encephalopathy

Это дубликат статьи: «Antibodies against Synthetic Fragments of the Prion Protein for Diagnostics of Bovine Spongiform Encephalopathy»

Seven peptides matching fragments of the prion protein and containing from 17 to 31 amino acid residues were synthesized to obtain antibodies for diagnostics of bovine spongiform encephalopathy. Rabbits were immunized with either free peptides or peptide-protein conjugates to result in sera with a high level of antipeptide antibodies. Immunohistochemical assay revealed sera against four free peptides and a protein-peptide conjugate, which effectively bind to the pathogenic isoform of the prion protein in brain tissue preparations from cattle afflicted with bovine spongiform encephalopathy and do not interact with normal brain preparations. The resulting antipeptide sera can be used in developing a diagnostic kit for bovine spongiform encephalopathy. The English version of the paper:.

Volpina OM, Zhmak MN, Oboznaya MB, Titova MA, Koroev DO, Volkova TD, Egorov AA, Rybakov SS, Ivanov VT

IBCH: 5643
Кол-во цитирований на 12.2023: 3
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