New version of sequential DNA and polyamide solid phase synthesis is applied to the preparation of PNA and PNA-DNA conjugates bearing free 3'-end. N-Mmt protected uracil and thymine PNA monomers are obtained in high yields. Novel support derived from TSK Gel Toyopearl® and efficient coupling procedure with 2,4,6-triisopropylbenzenesulfonyloxy-1-benzotriazole (TPSOBt) are also developed. Preliminary results of biological testing of prepared compounds are reported.