Cell Cycle, 2017, 16(20):1979-1991

Involvement of the specific nucleolar protein SURF6 in regulation of proliferation and ribosome biogenesis in mouse NIH/3T3 fibroblasts

The nucleolar proteins which link cell proliferation to ribosome biogenesis are regarded to be potentially oncogenic. Here, in order to examine the involvement of an evolutionary conserved nucleolar protein SURF6/Rrp14 in proliferation and ribosome biogenesis in mammalian cells, we established stably transfected mouse NIH/3T3 fibroblasts capable of conditional overexpression of the protein. Cell proliferation was monitored in real-time, and various cell cycle parameters were quantified based on flow cytometry, Br-dU-labeling and conventional microscopy data. We show that overexpression of SURF6 accelerates cell proliferation and promotes transition through all cell cycle phases. The most prominent SURF6 pro-proliferative effects include a significant reduction of the population doubling time, from 19.8 ± 0.7 to 16.2 ± 0.5 hours (t-test, p

Moraleva A, Magoulas C, Polzikov M, Hacot S, Mertani HC, Diaz JJ, Zatsepina O

IBCH: 6459
Ссылка на статью в журнале: https://www.tandfonline.com/doi/full/10.1080/15384101.2017.1371880
Кол-во цитирований на 03.2024: 11
Данные статьи проверены модераторами 2017-10-18