Bioorg Khim, 1995, 21(2):117-123

Dependence of the level of gene expression in E. coli on the structure of the translation initiation segment (TIR). I. Primary structure of TIR

The comparison of expression levels of two genes-interleukin-3 (il3) and epidermal growth factor connected to leader peptide of OmpF (lompegf)-was carried out using specially constructed plasmids contained various structures of translational enhancers. It was shown that besides already known binding sites from mRNA translation initiation region (TIR) to 16S rRNA (SD, UB1 and DB), there is additional binding site of TIR disposed from -30 to -60 nt upstream of start codon AUG (UB2) having significant increasing effect on translation initiation and correspondingly on expression level.

Gurevich AI, Esipov RS, Kachalina TA, Kaliushin AL, Korosteleva MD

IBCH: 7223
Кол-во цитирований на 01.2024: 3
Информация пока не проверена модераторами