Mol Biol Rep, 2007, 34(3):155-160

The nucleolar protein SURF-6 is essential for viability in mouse NIH/3T3 cells

SURF-6 is a bona fide nucleolar protein comprising an evolutionary conserved family that extends from human to yeast. The expression of the mammalian SURF-6 has been recently found to be regulated during the cell cycle. In order to determine the importance of SURF-6 in mammalian cells, we applied the Tet-On system to regulate conditionally, in response to tetracycline, the expression of an antisense RNA (asRNA) that targets Surf-6 mRNA in mouse NIH/3T3 cells. Induced Surf-6 asRNA caused an effective depletion of SURF-6 protein resulted in cell death and in an apparent arrest in the G1 phase of the cell cycle. These results provide for the first time evidence that expression of SURF-6 is essential for mammalian cell viability, and suggest that SURF-6 might participate in the progression of cell cycle. © 2006 Springer Science+Business Media, Inc.

Polzikov M, Magoulas C, Zatsepina O

IBCH: 996
Ссылка на статью в журнале: http://link.springer.com/10.1007/s11033-006-9028-4
Кол-во цитирований на 11.2023: 11
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