Russ. J. Bioorganic Chem., 2005, 31(6):563-566

Enzymatic synthesis of bis(5′-nucleosidyl) tetra- and triphosphates

The total fraction of aminoacyl-tRNA synthases from Escherichia coli has been shown to catalyze the synthesis of the bis(5′-nucleosidyl) oligophosphates Ap4AZT, Ap4d4T, Ap43TC, and Ap4ACV, as well as Ap3AZT and Ap3d4T, from [α-32P] ATP and the corresponding nucleoside-5′-tri(or di)phosphate. The resulting compounds, characterized by HPLC, are resistant to alkaline phosphatase. Ap4AZT, Ap4d4T, and Ap43TC are formed with approximately equal efficiency, whereas the efficiencies of the synthesis of Ap4ACV, Ap3AZT, and Ap3d4T are three- to fivefold lower. © 2005 Pleiades Publishing, Inc.

Skoblov AY, Yasko MV, Murabuldaev AM, Kukhanova MK, Skoblov YS

IBCH: 1142
Ссылка на статью в журнале: http://link.springer.com/10.1007/s11171-005-0077-6
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