Russ. J. Bioorganic Chem., 2004, 30(5):431-435

Recombinant thymosin α 1

An artificial gene encoding thymosin α1was obtained by chemoenzymatic synthesis and cloned into Escherichia coli. An expressing recombinant plasmid containing the hybrid protein gene, which encodes amino acid sequences of thymosin α1and the Saccharomyces cerevisiae intein Sce VMA, was constructed. The expression of the hybrid protein from the resulting hybrid gene in E. coli, the properties of the resulting hybrid protein, and the conditions for its nonenzymatic cleavage to thymosin α1were studied.

IBCH: 1366
Ссылка на статью в журнале: http://link.springer.com/10.1023/B:RUBI.0000043785.41076.56
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