FEBS Lett, 2000, 479(3):127-130

Novel fluorescent protein from Discosoma coral and its mutants possesses a unique far-red fluorescence

A novel gene for advanced red-shifted protein with an emission maximum at 593 nm was cloned from Discosoma coral. The protein, named dsFP593, is highly homologous to the recently described GFP-like protein drFP583 with an emission maximum at 583 nm. Using the remarkable similarity of the drFP583 and dsFP593 genes, we performed a 'shuffling' procedure to generate a pool of mutants consisting of various combinations of parts of both genes. One 'hybrid gene' was chosen for subsequent random mutagenesis, which resulted in a mutant variant with a uniquely red-shifted emission maximum at 616 nm. Copyright (C) 2000 Federation of European Biochemical Societies.

Fradkov AF, Chen Y, Ding L, Barsova EV, Matz MV, Lukyanov SA

IBCH: 1540
Ссылка на статью в журнале: http://doi.wiley.com/10.1016/S0014-5793%2800%2901895-0
Кол-во цитирований на 11.2023: 126
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