Nucleic Acids Res, 1996, 24(19):3748-3755

A convenient approach to the synthesis of trinucleotide phosphoramidites-synthons for the generation of oligonucleotide/peptide libraries

Trinucleotide phosphoramidites that correspond to the codons of all 20 amino acids were synthesized in high yield in 5g scale. Precursors of those amidites-trinucleotide phosphotriesters-have been prepared using the phosphotriester approach without protection of the 3'-hydroxyl function. The structures of trinucleotide phosphotriesters and intermediates were confirmed by1H- and31P-NMR spectra, mass-spectra and by analysis of SPDE-hydrolysates of deprotected preparations. Purity of the target products has been confirmed by test reactions. The synthons have been used for automated synthesis of oligonucleotides and corresponding libraries by a phosphite-triester approach. A 54mer, containing 12 randomized internal bases, and a 72mer with 24 internal randomized bases have been synthesized.

Kayushin AL, Korosteleva MD, Miroshnikov AI, Kosch W, Zubov D, Piel N

IBCH: 2088
Ссылка на статью в журнале: https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/24.19.3748
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