The relative positions of the ZNF91 gene (exon-intron organization) and the HERV-K human endogenous virus long terminal repeat (LTR), which was earlier found to be located in the ZNF91 gene locus, on the EcoRI restriction metric map of the human chromosome 19 were determined with a high resolution. The direction of the ZNF91 gene transcription relative to the chromosome 19 telomeres was determined. The HERV-K LTR was localized to the ZNF91 gene intron 19. The role of retroviral sequences in the evolution of the ZNF91 gene family is discussed.