Colloids Surf B Biointerfaces, 2018, 167:328-336

Effect of lipopeptide structure on gene delivery system properties: Evaluation in 2D and 3D in vitro models

Development of efficient biodegradable, environmentally responsive, biocompatible and non-toxic delivery system is needed for efficient gene delivery. As well known, properties of the vehicle are determined by the structure of carrier components. The aim of the current study was to estimate in vitro transfection efficacy of aliphatic di-, tri- and tetrapeptide-based cationic lipoplexes loaded with siRNA in function of a number of cationic groups using 2D (monolayer culture) and 3D (multicellular tumor spheroids) in vitro models. Physicochemical properties and cytotoxicity of the liposomes were found to be dependent upon a number of amino acid derivatives in an amphiphilic polar head. Uptake of liposomes loaded with nucleic acid (lipoplexes) and their localization in HEK293T cells was studied by confocal microscopy. The liposomes based on lipotripeptides had the highest transfection efficiency which was 20-fold higher than those fabricated from lipotetrapeptides.

Koloskova OO, Gileva AM, Drozdova MG, Grechihina MV, Suzina NE, Budanova UA, Sebyakin YL, Kudlay DA, Shilovskiy IP, Sapozhnikov AM, Kovalenko EI, Markvicheva EA, Khaitov MR

IBCH: 3024
Ссылка на статью в журнале: https://linkinghub.elsevier.com/retrieve/pii/S0927776518302078
Кол-во цитирований на 02.2024: 11
Данные статьи проверены модераторами 2018-07-01