Opt Lett, 2016, 41(23):5563-5566

Fiber-optic electron-spin-resonance thermometry of single laser-activated neurons

Optically detected electron spin resonance in fiber-coupled nitrogen-vacancy (NV) centers of diamond is used to demonstrate a fiber-optic quantum thermometry of individual thermogenetically activated neurons. Laser-induced temperature variations read out from single neurons with the NV-diamond fiber sensor are shown to strongly correlate with the fluorescence of calcium-ion sensors, serving as online indicators of the inward Ca2+current across the cell membrane of neurons expressing transient receptor potential (TRP) cation channels. Local laser heating above the TRP-channel activation threshold is shown to reproducibly evoke robust action potentials, visualized by calcium-ion-sensor-aided fluorescence imaging and detected as prominent characteristic waveforms in the time-resolved response of fluorescence Ca2+sensors.

Lanin AA, Fedotov IV, Ermakova YG, Sidorov-Biryukov DA, Fedotov AB, Hemmer P, Belousov VV, Zheltikov AM

IBCH: 3578
Ссылка на статью в журнале: https://www.osapublishing.org/abstract.cfm?URI=ol-41-23-5563
Кол-во цитирований на 09.2023: 25
Данные статьи проверены модераторами 2016-12-01