Res J Pharm Biol Chem Sci, 2016, 7(5):3105-3110

Modified CTAB protocol for the isolation of high-quality RNA from silver and downy birch leaves

Obtaining high quality RNA preparations is an important initial step in transcriptomic studies. Isolation of RNAs from tissues of woody plants, birch for instance, is hampered by the presence of polyphenols and polysaccharides, which make RNA samples useless in downstream applications. To overcome this obstacle, a modified CTAB protocol was proposed for the RNA isolation from birch leaves. This protocol includes acetone treatment of a plant issue. The protocol is applicable to the two birch species, silver birch and downy birch. It allows obtaining high-quality RNA preparations that feature high purity and high concentration along with low extent of degradation, in a highly reproducible manner.

IBCH: 3715
Кол-во цитирований на 12.2023: 0
Данные статьи проверены модераторами 2016-09-01