PLoS One, 2015, 10(12):e0145287

KillerOrange, a genetically encoded photosensitizer activated by blue and green light

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Genetically encoded photosensitizers, proteins that produce reactive oxygen species when illuminated with visible light, are increasingly used as optogenetic tools. Their applications range from ablation of specific cell populations to precise optical inactivation of cellular proteins. Here, we report an orange mutant of red fluorescent protein KillerRed that becomes toxic when illuminated with blue or green light. This new protein, KillerOrange, carries a tryptophanbased chromophore that is novel for photosensitizers.We show that KillerOrange can be used simultaneously and independently from KillerRed in both bacterial and mammalian cells offering chromatic orthogonality for light-activated toxicity.

IBCH: 3764
Ссылка на статью в журнале: http://dx.plos.org/10.1371/journal.pone.0145287
Кол-во цитирований на 12.2023: 54
Информация пока не проверена модераторами