Bioorg Med Chem, 2015, 23(20):6749-6756

Solid- and solution-phase synthesis and application of R6G dual-labeled oligonucleotide probes

All Rights Reserved. A novel N-TFA-protected carboxyrhodamine 6G (R6G) phosphoramidite was synthesized for use in an automated DNA synthesis to prepare 5′-labeled oligonucleotides. Deprotection and purification conditions were optimized for 5′-labeled and dual-labeled oligonucleotide probes. As an alternative we synthesized an azide derivative of R6G for CuAAC post-synthetic oligonucleotide labeling. Dual-labeled probes obtained by both methods showed the same efficacy in a quantitative PCR assay. R6G-labeled probes demonstrated superior properties in a qPCR assay in comparison with alternative HEX, JOE and SIMA dyes due to more efficient fluorescence quenching by BHQ-1. We successfully used R6G dual-labeled probes for rotavirus genotyping.

Skoblov AY, Vichuzhanin MV, Farzan VM, Veselova OA, Konovalova TA, Podkolzin AT, Shipulin GA, Zatsepin TS

IBCH: 3842
Ссылка на статью в журнале: https://linkinghub.elsevier.com/retrieve/pii/S0968089615300237
Кол-во цитирований на 11.2023: 7
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