Photonics Lasers Med, 2016, 5(2):113-121

Hydrogen peroxide detection in viable and apoptotic tumor cells under action of cisplatin and bleomycin

Objective: A flow cytometric approach is proposed to assess the hydrogen peroxide (H2O2) level under chemotherapy action separately in viable and apoptotic tumor cells. Materials and methods: For studying the involvement of H2O2in the process of cell death, the genetically encoded fluorescent sensor HyPer2, apoptosis marker PE Annexin V and vital dye 7-AAD were employed. The approach was used for testing the capacity of two cytotoxic drugs, cisplatin and bleomycin, to change the intracellular H2O2concentration, depending on the stage of cell death. Results: An increase in HyPer2 fluorescence has been revealed in cells undergoing apoptosis under cisplatin action. This finding indicates that accumulation of H2O2accompanies the cisplatin-induced apoptotic reaction. HyPer2 response was also revealed in negative to PE Annexin V viable cells which can be explained either by participation of H2O2in the earliest stages of apoptosis or in a cell response to a non-fatal injury. Under bleomycin action, neither an apoptotic reaction nor changes of fluorescence intensity HyPer2 were detected, allowing one to assume that H2O2is not involved in the reaction of tumor cells to bleomycin. Conclusion: The proposed approach can be used for studying the mechanisms of cell death under action of any types of antitumor drugs.

Belova AS, Orlova AG, Balalaeva IV, Antonova NO, Maslennikova AV, Mishina NM, Zagaynova EV

IBCH: 3937
Кол-во цитирований на 03.2024: 8
Данные статьи проверены модераторами 2016-04-06