Russ. J. Bioorganic Chem., 2011, 37(4):464-471

The use of duplex-specific crab nuclease for rapid analysis of single-nucleotide polymorphisms and the detection of dna targets in complex PCR products

A rapid method for single-nucleotide polymorphism analysis and the detection of target clones after cloning of complex PCR products based on the use of duplex-specific crab nuclease and a universal fluorescent probe has been developed. The method is an alternative to the labor-intensive procedures of clone screening employing radioactively labeled probes, gel-based restriction analysis, and costly sequencing. The efficiency of the novel method has been demonstrated in a range of model systems. © Pleiades Publishing, Ltd., 2011.

IBCH: 4854
Ссылка на статью в журнале: http://link.springer.com/10.1134/S1068162011040121
Кол-во цитирований на 10.2023: 1
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