Mol Biol, 2013, 47(4):524-529

Molecular genetic analysis of DNA polymerase and thymidine kinase genes of a HSV-1 population using an MPS technology

Real-time PCR was used to determine the ratio of viral and host DNA in lysates of Vero cells infected with HSV-1 strain L2. The number of virus copies reached a maximum after 24 h of incubation. Total isolated DNA was sequenced using the massively parallel sequencing technique on an Ion Torrent apparatus. Nucleotide sequences of thymidine kinase (UL23) and DNA polymerase (UL30) genes of a HSV-1 L2 population were determined; their primary structures were compared to those of other standard HSV-1 strains, KOS and 17. The detected differences between the UL23 and UL30 sequences of L2 and reference strains KOS and 17 were unimportant because these substitutions did not affect the conserved gene regions. © 2013 Pleiades Publishing, Ltd.

Guskova AA, Skoblov MY, Lavrov AV, Zubtsov DA, Andronova VL, Goldshtein DV, Galegov GA, Skoblov YS

IBCH: 5009
Ссылка на статью в журнале: http://link.springer.com/10.1134/S0026893313040067
Кол-во цитирований на 03.2024: 0
Информация пока не проверена модераторами