Preparation of pure monoclonal antibody to interleukin-2 by cultivation of hybridoma cells entrapped in novel composite hydrogel beads
A simple and reliable technique was developed to prepare pure monoclonal antibody (MAb) to Interleukin-2 using cells entrapped in novel composite poly(N-vinyl caprolactam)-calcium alginate beads. Flow cytometry was applied to study cell size and cell cytoplasm granularity distribution. Maximum MAb production by the get-entrapped cells in serum free medium was 2-3-fold higher compared to free suspension culture in serum containing medium. The only contaminating protein in culture supernatant was transferrin at 5% w/v.