J Biotechnol, 2016, 231:S69

Features of cultivation of mesenchymal stromal cells from bone marrow of cattle on gelatin cryogel

A significant limitation for a wider use of mesenchymal stromal cells from the bone marrow of cattle (cMSC BM) is their heterogeneity and genomic instability. The problem can be solved by the development of special cell carriers from polymeric cryogels to stabilize the conditions of cultivation MSC in a given direction to produce monocultures or imitations of tissue in vitro. If the problem of process management of differentiation MSC BM will be solved, it will be possible to obtain the necessary number of homogeneous biomass for therapy and transplantation. In the current work we show the possibility to obtain multipotential cMSC able to differentiate in osteogenic direction when cultured on gelatin cryogels. According to our experimental data, cMSC BM kept on surface and inside the cryogel are able to actively proliferate and migrate into the environment, which makes them more accessible for applications in regenerative medicine. Cultivation of cMSCBMon gelatin cryogels does not require any additional efforts and special additives in the form of growth factors, except of mitogenic activators of serum. Thus, gelatin cryogels represent a convenient and rather simple 3D matrix for MSC culturing and growing osteogenic tissue for therapeutic purposes.   http://www.sciencedirect.com/science/article/pii/S016816561630517X

Khovankina AV, Mezhevikina LM, Коsоvskу GU

IBCH: 6712
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Данные статьи проверены модераторами 2016-10-08