A new chemical method of synthesis of modified nucleoside [ 32P]phosphates
A simple method of chemical phosphorylation for modified nucleosides with [32P]orthophosphoric acid in the presence of BrCN is described. The yields of 5′-[32P]nucleoside monophosphates achieved are 50-65% at nominal specific radioactivities of ca. 1000 Ci/mmol. The mechanism of phosphorylation in the presence of heterocyclic amines is studied. Copyright © 2008 John Wiley & Sons, Ltd.