Каюшин Алексей Львович

Кандидат химических наук

Старший научный сотрудник (Лаборатория биотехнологии)

Тел.: +7 (495) 3366833

Эл. почта: kaushin@ibch.ru

Избранные публикации

  1. Kayushin A., Korosteleva M., Miroshnikov A. (2009). Large-scale solid-phase preparation of 3'-unprotected trinucleotide phosphotriesters--precursors for synthesis of trinucleotide phosphoramidites. Nucleosides Nucleotides Nucleic Acids 19 (10-12), 1967–76 [+]

    The approach to large-scale solid-phase synthesis of 3'-unprotected trinucleotide phosphotriesters has been developed. The trinucleotides have been synthesized in 5 g scale by phosphotriester approach using CPG with pore size 70A. Total yield of target products was 75-90%. The molar extinctions of trinucleotides at various wave-lengths were calculated; the experimental UV-spectra of trinucleotides show a good agreement with theoretical ones. The trinucleotides synthesized were used for synthesis of trinucleotide phosphoramidites - synthons for generation of DNA/peptide libraries.

  2. Yagodkin A., Azhayev A., Roivainen J., Antopolsky M., Kayushin A., Korosteleva M., Miroshnikov A., Randolph J., Mackie H. (2007). Improved synthesis of trinucleotide phosphoramidites and generation of randomized oligonucleotide libraries. Nucleosides Nucleotides Nucleic Acids 26 (5), 473–97 [+]

    A new method to produce a set of 20 high quality trinucleotide phosphoramidites on a 5-10 g scale each was developed. The procedure starts with condensation reactions of P-components with N-acyl nucleosides, bearing the 3 '-hydroxyl function protected with 2-azidomethylbenzoyl, to give fully protected dinucleoside phosphates 13. Upon cleavage of dimethoxytrityl group from 13, dinucleoside phosphates 16 are initially transformed into trinucleoside diphosphates 19 and then the 2-azidomethylbenzoyl is selectively removed under neutral conditions to generate trinucleoside diphosphates 5 in excellent yield. Subsequent 3 '-phosphitylation affords target trinucleotide phosphoramidites 7. When mutagenic oligonucleotides are synthesized employing mixtures of building blocks 7 as well as following the new synthetic protocol, representative oligonucleotide libraries are generated in good yields.