Microscopic Imaging of Epigenetic Landscape - A Universal Platform to Study Epigenetic Changes at Single Cell Level

We have developed a unique phenotypic high throughput screening platform (MIEL), which interrogates the epigenetic landscape at both population and single cell level using image derived features and machine learning. We have demonstrated the utility of such approach to identify epigenetic signature of differentiated primary glioblastoma cells, which enables high content drug screening for differentiation-inducing compounds (Farhy et al., biorxiv 2018). More recently, we applied MIEL to identify epigenetically toxic environmental compounds and epigenetic differences associated with aging. In close collaboration with Dr. K. Lukyanov at IBCh we have developed Genetically Encoded Epigenetic Probes (GEEPs) enabling for the first time comprehensive imaging of epigenetic alterations in live cells.

february 6, 2019