Shemyakin & Ovchinikov Institute of Bioorganic Chemistry
Laboratory of Molecular Technologies
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Professional appointments
Scientific background

Konstantin A. Lukyanov

Born: March 18, 1970, Moscow, Russia (USSR)
Citizenship: Russia
Business address:
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Micklukho-Maklaya 16/10, 117871, Moscow, Russia;
tel: 7(495) 429-80-20
fax: 7(495) 330-70-56

Senior scientist, Laboratory of Molecular Technologies (prev. Laboratory of Genes for Regeneration), Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.

1986-1991 Molecular Biology Department, Moscow State University (Moscow, Russia)

1993-1998 Junior scientist. Laboratory of Structure and Function of Human Genes, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
1999-present Research scientist, then Senior scientist. Laboratory of Molecular Technologies (prev. Laboratory of Genes for Regeneration), Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia.

1998 Ph.D. thesis: "Effect of selective suppression of Polymerase Chain Reaction (PCR): applications in molecular biology".
2006 Dr.Sc. thesis: "Fluorescent proteins for experimental biology: natural diversity and directed changes of spectral properties".

Area of expertise: PCR-based methods for gene searching; GFP-like fluorescent and chromoproteins from Anthozoa species.

  1. Yampolsky IV, Kislukhin AA, Amatov TT, Shcherbo D, Potapov VK, Lukyanov S, Lukyanov KA.
    Synthesis and properties of the red chromophore of the green-to-red photoconvertible fluorescent protein Kaede and its analogs.
    Bioorg Chem. 2008 Apr;36(2):96-104
    PMID: 18262585

  2. Mishin AS, Subach FV, Yampolsky IV, King W, Lukyanov KA, Verkhusha VV.
    The First Mutant of the Aequorea victoria Green Fluorescent Protein That Forms a Red Chromophore.
    Biochemistry, 2008
    PMID: 18366185

  3. Cox LJ, Hengst U, Gurskaya NG, Lukyanov KA, Jaffrey SR.
    Intra-axonal translation and retrograde trafficking of CREB promotes neuronal survival.
    Nat Cell Biol. 2008 Feb;10(2):149-59
    PMID: 18193038

  4. Lukyanov S, Lukyanov K, Gurskaya N, Bogdanova E, Buzdin A.
    Selective suppression of polymerase chain reaction and its most popular applications.
    In: Nucleic Acids Hybridization: Modern Applications. Buzdin, Anton; Lukyanov, Sergey (Eds.) Hardcover, 2007; 29-51 ISBN: 978-1-4020-6039-7

  5. Chudakov DM, Lukyanov S, Lukyanov KA.
    Tracking intracellular protein movements using photoswitchable fluorescent proteins PS-CFP2 and Dendra2.
    Nat Protoc. 2007;2(8):2024-32
    PMID: 17703215

  6. Shcherbo D, Merzlyak EM, Chepurnykh TV, Fradkov AF, Ermakova GV, Solovieva EA, Lukyanov KA, Bogdanova EA, Zaraisky AG, Lukyanov S, Chudakov DM.
    Bright far-red fluorescent protein for whole-body imaging.
    Nat Methods. 2007 Sep;4(9):741-6
    PMID: 17721542

  7. Merzlyak EM, Goedhart J, Shcherbo D, Bulina ME, Shcheglov AS, Fradkov AF, Gaintzeva A, Lukyanov KA, Lukyanov S, Gadella TW, Chudakov DM.
    Bright monomeric red fluorescent protein with an extended fluorescence lifetime.
    Nat Methods. 2007 Jul;4(7):555-7
    PMID: 17572680

  8. Chudakov DM, Lukyanov S, Lukyanov KA.
    Using photoactivatable fluorescent protein Dendra2 to track protein movement.
    Biotechniques. 2007 May;42(5):553, 555, 557 passim
    PMID: 17515192

  9. Zhang L, Gurskaya NG, Merzlyak EM, Staroverov DB, Mudrik NN, Samarkina ON, Vinokurov LM, Lukyanov S, Lukyanov KA.
    Method for real-time monitoring of protein degradation at the single cell level.
    Biotechniques. 2007 Apr;42(4):446, 448, 450
    PMID: 17489230

  10. Schuttrigkeit TA, Feilitzsch T, Kompa C, Lukyanov KA, Savitsky A, Rosch N, Voityuk AA, Michel-Beyerle ME.
    Femtosecond study of superlinear light induced fluorescence increase of the coral protein asFP595.
    Chem Phys. 2006;323:149-60

  11. Lukyanov KA, Chudakov DM, Fradkov AF, Labas YA, Matz MV, Lukyanov S.
    Discovery and properties of GFP-like proteins from nonbioluminescent anthozoa.
    Methods Biochem Anal. 2006;47:121-38
    PMID: 16335712

  12. Bulina ME, Lukyanov KA, Britanova OV, Onichtchouk D, Lukyanov S, Chudakov DM.
    Chromophore-assisted light inactivation (CALI) using the phototoxic fluorescent protein KillerRed.
    Nat Protoc. 2006;1(2):947-53
    PMID: 17406328

  13. Evdokimov AG, Pokross ME, Egorov NS, Zaraisky AG, Yampolsky IV, Merzlyak EM, Shkoporov AN, Sander I, Lukyanov KA, Chudakov DM.
    Structural basis for the fast maturation of Arthropoda green fluorescent protein.
    EMBO Rep. 2006 Oct;7(10):1006-12
    PMID: 16936637

  14. Chudakov DM, Chepurnykh TV, Belousov VV, Lukyanov S, Lukyanov KA.
    Fast and precise protein tracking using repeated reversible photoactivation.
    Traffic. 2006 Oct;7(10):1304-10
    PMID: 16889652

  15. Gurskaya NG, Verkhusha VV, Shcheglov AS, Staroverov DB, Chepurnykh TV, Fradkov AF, Lukyanov S, Lukyanov KA.
    Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light.
    Nat Biotechnol. 2006 Apr;24(4):461-5
    PMID: 16550175

  16. Belousov VV, Fradkov AF, Lukyanov KA, Staroverov DB, Shakhbazov KS, Terskikh AV, Lukyanov S.
    Genetically encoded fluorescent indicator for intracellular hydrogen peroxide.
    Nat Methods. 2006 Apr;3(4):281-6
    PMID: 16554833

  17. Bulina ME, Chudakov DM, Britanova OV, Yanushevich YG, Staroverov DB, Chepurnykh TV, Merzlyak EM, Shkrob MA, Lukyanov S, Lukyanov KA.
    A genetically encoded photosensitizer.
    Nat Biotechnol. 2006 Jan;24(1):95-9
    PMID: 16369538

  18. Lukyanov KA, Chudakov DM, Fradkov AF, Labas YA, Matz MV and Lukyanov SA.
    Discovery and properties of GFP-like proteins from non-bioluminescent Anthozoa.
    In: Green fluorescent protein: properties and applications. Chalfie, M., Kain, S., (Eds). Willey-Liss, New York, 2005; 121-38

  19. Chudakov DM, and Lukyanov KA.
    Using photoactivatable GFPs to study protein dynamics and function.
    In: Jorde, L.B., Little, P.F.R., Dunn, M.J. and Subramaniam, S. (Eds), Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. John Wiley & Sons Ltd: Chichester, 2005; 2129-37

  20. Chudakov DM, Lukyanov S, Lukyanov KA.
    Fluorescent proteins as a toolkit for in vivo imaging.
    Trends Biotechnol. 2005 Dec;23(12):605-13
    PMID: 16269193

  21. Shkrob MA, Yanushevich YG, Chudakov DM, Gurskaya NG, Labas YA, Poponov SY, Mudrik NN, Lukyanov S, Lukyanov KA.
    Far-red fluorescent proteins evolved from a blue chromoprotein from Actinia equina.
    Biochem J. 2005 Dec 15;392(Pt 3):649-54
    PMID: 16164420

  22. Lukyanov KA, Chudakov DM, Lukyanov S, Verkhusha VV.
    Innovation: Photoactivatable fluorescent proteins.
    Nat Rev Mol Cell Biol. 2005 Nov;6(11):885-91
    PMID: 16167053

  23. Yampolsky IV, Remington SJ, Martynov VI, Potapov VK, Lukyanov S, Lukyanov KA.
    Synthesis and properties of the chromophore of the asFP595 chromoprotein from Anemonia sulcata.
    Biochemistry. 2005 Apr 19;44(15):5788-93
    PMID: 15823037

  24. Yanushevich YG, Shagin DA, Fradkov AF, Shakhbazov KS, Barsova EV, Gurskaya NG, Labas YA, Matz MV, Lukyanov KA, Lukyanov SA.
    Spectral diversity among the members of the family of Green Fluorescent Protein in hydroid jellyfish (Cnidaria, Hydrozoa).
    Bioorg Khim. 2005 Jan-Feb;31(1):49-53 (in Russian)
    PMID: 15787213

  25. Remington SJ, Wachter RM, Yarbrough DK, Branchaud B, Anderson DC, Kallio K, Lukyanov KA.
    zFP538, a yellow-fluorescent protein from Zoanthus, contains a novel three-ring chromophore.
    Biochemistry. 2005 Jan 11;44(1):202-12
    PMID: 15628861

  26. Verkhusha VV, Chudakov DM, Gurskaya NG, Lukyanov S, Lukyanov KA.
    Common pathway for the red chromophore formation in fluorescent proteins and chromoproteins.
    Chem Biol. 2004;11(6):845-54
    PMID: 15217617

  27. Chudakov DM, Verkhusha VV, Staroverov DB, Souslova EA, Lukyanov S, Lukyanov KA.
    Photoswitchable cyan fluorescent protein for protein tracking.
    Nat Biotechnol. 2004 Nov;22(11):1435-9
    PMID: 15502815

  28. Bulina ME, Lukyanov KA, Yampolsky IV, Chudakov DM, Staroverov DB, Shcheglov AS, Gurskaya NG, Lukyanov S.
    New class of blue animal pigments based on Frizzled and Kringle protein domains.
    J Biol Chem. 2004 Oct 15;279(42):43367-70
    PMID: 15297465

  29. Belov GA, Lidsky PV, Mikitas OV, Egger D, Lukyanov KA, Bienz K, Agol VI.
    Bidirectional increase in permeability of nuclear envelope upon poliovirus infection and accompanying alterations of nuclear pores.
    J Virol. 2004 Sep;78(18):10166-77
    PMID: 15331749

  30. Shagin DA, Barsova EV, Yanushevich YG, Fradkov AF, Lukyanov KA, Labas YA, Semenova TN, Ugalde JA, Meyers A, Nunez JM, Widder EA, Lukyanov SA, Matz MV.
    GFP-like proteins as ubiquitous Metazoan superfamily: evolution of functional features and structural complexity.
    Mol Biol Evol. 2004 May;21(5):841-50
    PMID: 14963095

  31. Verkhusha VV, Lukyanov KA.
    The molecular properties and applications of Anthozoa fluorescent proteins and chromoproteins.
    Nat Biotechnol. 2004 Mar;22(3):289-96
    PMID: 14990950

  32. Verkhusha VV, Matz MV, Sakurai T, Lukyanov KA.
    GFP-like fluorescent proteins and chromoproteins of the class Anthozoa.
    In: Protein structures: kaleidoscope of structural properties and functions. Ed. Uversky V.N., Research Signpost, Kerala, India, 2003; 405-39

  33. Chudakov DM, Lukyanov KA.
    Use of green fluorescent protein (GFP) and its homologs for in vivo protein motility studies.
    Biochemistry (Mosc). 2003 Sep;68(9):952-7
    PMID: 14606935

  34. Yanushevich YG, Gurskaya NG, Staroverov DB, Lukyanov SA, Lukyanov KA.
    A natural fluorescent protein that changes its fluorescence during maturation.
    Bioorg Khim. 2003 Jul-Aug;29(4):356-60 (in Russian)
    PMID: 12947756

  35. Gurskaya NG, Fradkov AF, Pounkova NI, Staroverov DB, Bulina ME, Yanushevich YG, Labas YA, Lukyanov S, Lukyanov KA.
    A colourless green fluorescent protein homologue from the non-fluorescent hydromedusa Aequorea coerulescens and its fluorescent mutants.
    Biochem J. 2003 Jul 15;373(Pt 2):403-8
    PMID: 12693991

  36. Bulina ME, Verkhusha VV, Staroverov DB, Chudakov DM, Lukyanov KA.
    Hetero-oligomeric tagging diminishes non-specific aggregation of target proteins fused with Anthozoa fluorescent proteins.
    Biochem J. 2003 Apr 1;371(Pt 1):109-14
    PMID: 12472468

  37. Chudakov DM, Belousov VV, Zaraisky AG, Novoselov VV, Staroverov DB, Zorov DB, Lukyanov S, Lukyanov KA.
    Kindling fluorescent proteins for precise in vivo photolabeling.
    Nat Biotechnol. 2003 Feb;21(2):191-4
    PMID: 12524551

  38. Chudakov DM, Feofanov AV, Mudrik NN, Lukyanov S, Lukyanov KA.
    Chromophore environment provides clue to kindling fluorescent protein riddle.
    J Biol Chem. 2003 Feb 28;278(9):7215-9
    PMID: 12496281

  39. Fradkov AF, Verkhusha VV, Staroverov DB, Bulina ME, Yanushevich YG, Martynov VI, Lukyanov S, Lukyanov KA.
    Far-red fluorescent tag for protein labelling.
    Biochem J. 2002 Nov 15;368(Pt 1):17-21
    PMID: 12350221

  40. Matz MV, Lukyanov KA, Lukyanov SA.
    Family of the green fluorescent protein: journey to the end of the rainbow.
    Bioessays. 2002 Oct;24(10):953-9
    PMID: 12325128

  41. Shagin DA, Barsova EV, Bogdanova E, Britanova OV, Gurskaya N, Lukyanov KA, Matz MV, Pounkova NI, Usman NY, Kopantzev EP, Salo E, Lukyanov SA.
    Identification and characterization of a new family of C-type lectin-like genes from planaria Girardia tigrina.
    Glycobiology. 2002 Aug;12(8):463-72
    PMID: 12145187

  42. Yanushevich YG, Bulina ME, Gurskaya NG, Savitskii AP, Lukyanov KA.
    Key amino acid residues responsible for the color of green and yellow fluorescent proteins from the coral polyp Zoanthus.
    Bioorg Khim. 2002 Jul-Aug;28(4):303-7 (in Russian)
    PMID: 12197386

  43. Bulina ME, Chudakov DM, Mudrik NN, Lukyanov KA.
    Interconversion of Anthozoa GFP-like fluorescent and non-fluorescent proteins by mutagenesis.
    BMC Biochem. 2002 Apr 24;3:7
    PMID: 11972899

  44. Labas YA, Gurskaya NG, Yanushevich YG, Fradkov AF, Lukyanov KA, Lukyanov SA, Matz MV.
    Diversity and evolution of the green fluorescent protein family.
    Proc Natl Acad Sci U S A. 2002 Apr 2;99(7):4256-61
    PMID: 11929996

  45. Yanushevich YG, Staroverov DB, Savitsky AP, Fradkov AF, Gurskaya NG, Bulina ME, Lukyanov KA, Lukyanov SA.
    A strategy for the generation of non-aggregating mutants of Anthozoa fluorescent proteins.
    FEBS Lett. 2002 Jan 30;511(1-3):11-4
    PMID: 11821040

  46. Gurskaya NG, Savitsky AP, Yanushevich YG, Lukyanov SA, Lukyanov KA.
    Color transitions in coral's fluorescent proteins by site-directed mutagenesis.
    BMC Biochem. 2001;2:6
    PMID: 11459517

  47. Gurskaya NG, Fradkov AF, Terskikh A, Matz MV, Labas YA, Martynov VI, Yanushevich YG, Lukyanov KA, Lukyanov SA.
    GFP-like chromoproteins as a source of far-red fluorescent proteins.
    FEBS Lett. 2001 Oct 19;507(1):16-20
    PMID: 11682051

  48. Martynov VI, Savitsky AP, Martynova NY, Savitsky PA, Lukyanov KA, Lukyanov SA.
    Alternative cyclization in GFP-like proteins family. The formation and structure of the chromophore of a purple chromoprotein from Anemonia sulcata.
    J Biol Chem. 2001 Jun 15;276(24):21012-6
    PMID: 11259412

  49. Rebrikov DV, Britanova OV, Gurskaya NG, Lukyanov KA, Tarabykin VS, Lukyanov SA.
    Mirror orientation selection (MOS): a method for eliminating false positive clones from libraries generated by suppression subtractive hybridization.
    Nucleic Acids Res. 2000 Oct 15;28(20):E90
    PMID: 11024192

  50. Lukyanov KA, Fradkov AF, Gurskaya NG, Matz MV, Labas YA, Savitsky AP, Markelov ML, Zaraisky AG, Zhao X, Fang Y, Tan W, Lukyanov SA.
    Natural animal coloration can be determined by a nonfluorescent green fluorescent protein homolog.
    J Biol Chem. 2000 Aug 25;275(34):25879-82
    PMID: 10852900

  51. Panchin Y, Kelmanson I, Matz M, Lukyanov K, Usman N, Lukyanov S.
    A ubiquitous family of putative gap junction molecules.
    Curr Biol. 2000 Jun 29;10(13):R473-4
    PMID: 10898987

  52. Shagin DA, Lukyanov KA, Wagner LL, Matz MV.
    Regulation of average length of complex PCR product.
    Nucleic Acids Res. 1999 Sep 15;27(18):e23
    PMID: 10471753

  53. Lukyanov KA, Gurskaya NG, Bogdanova EA, Lukyanov SA.
    Selective suppression of polymerase chain reaction.
    Bioorg Khim. 1999 Mar;25(3):163-70 (in Russian)
    PMID: 10382038

  54. Lukyanov K.
    In vitro cloning: a method for PCR amplification of individual unknown DNA fragments suitable for direct sequencing.
    In: Gene Cloning and Analysis by RT-PCR. Ed. by P. Siebert and J. Larrick, BioTechniques Books, Natick, MA, 1998; 329-45

  55. Chen SS, Chenchik A, Lukyanov K, Siebert PD.
    Improved technique for walking in uncloned genomic DNA.
    In: Gene Cloning and Analysis by RT-PCR. Ed. by P. Siebert and J. Larrick, BioTechniques Books, Natick, MA, 1998; 289-304

  56. Fradkov AF, Lukyanov KA, Matz MV, Diatchenko LB, Siebert PD, Lukyanov SA.
    Sequence-independent method for in vitro generation of nested deletions for sequencing large DNA fragments.
    Anal Biochem. 1998 Apr 10;258(1):138-41
    PMID: 9527860

  57. Lukyanov KA, Lukyanov SA.
    In vitro cloning of DNA fragments by one polymerase chain reaction.
    Bioorg Khim. 1997 Nov;23(11):882-7 (in Russian)
    PMID: 9518428

  58. Markitantova IuV, Lukyanov KA, Kazanskaia OV, Mitashov VI, Lukyanov SA.
    An analysis of the expression of the genes containing the LeR-1 and VeR-1 sequences in the embryogenesis, regeneration and in the intact tissues of newts.
    Ontogenez. 1997 Jul-Aug;28(4):262-70 (in Russian)
    PMID: 9304090

  59. Lukyanov K, Diatchenko L, Chenchik A, Nanisetti A, Siebert P, Usman N, Matz M, Lukyanov S.
    Construction of cDNA libraries from small amounts of total RNA using the suppression PCR effect.
    Biochem Biophys Res Commun. 1997 Jan 13;230(2):285-8
    PMID: 9016767

  60. Lukyanov KA, Gurskaya NG, Matz MV, Khaspekov GL, Diatchenko LB, Chenchik AA, Il'evich-Stuchkov SG, Lukyanov SA.
    A method for obtaining the normalized cDNA libraries based on the effect of suppression of polymerase chain reaction.
    Bioorg Khim. 1996 Sep;22(9):686-90 (in Russian)
    PMID: 8999786

  61. Gurskaya NG, Diatchenko L, Chenchik A, Siebert PD, Khaspekov GL, Lukyanov KA, Wagner LL, Ermolaeva OD, Lukyanov SA, Sverdlov ED.
    Equalizing cDNA subtraction based on selective suppression of polymerase chain reaction: cloning of Jurkat cell transcripts induced by phytohemaglutinin and phorbol 12-myristate 13-acetate.
    Anal Biochem. 1996 Aug 15;240(1):90-7
    PMID: 8811883

  62. Gurskaya NG, Shagin DA, Lukyanov KA, Wagner LL, Shtutman MS, Musatkina EA, Moinova EV, Tatosian AG, Lukyanov SA, Sverdlov ED.
    Cloning cDNA for the ha-SDGF gene from a Syrian hamster cell line with increased metastatic potential using subtractive hybridization.
    Bioorg Khim. 1996 Jun;22(6):425-31 (in Russian)
    PMID: 8975671

  63. Diatchenko L, Lau YF, Campbell AP, Chenchik A, Moqadam F, Huang B, Lukyanov S, Lukyanov K, Gurskaya N, Sverdlov ED, Siebert PD.
    Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries.
    Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):6025-30
    PMID: 8650213

  64. Lukyanov KA, Matz MV, Bogdanova EA, Gurskaya NG, Lukyanov SA.
    Molecule by molecule PCR amplification of complex DNA mixtures for direct sequencing: an approach to in vitro cloning.
    Nucleic Acids Res. 1996 Jun 1;24(11):2194-5
    PMID: 8668555

  65. Lukyanov KA, Gurskaya NG, Kopantsev EP, Lukyanov SA.
    Selective amplification of evolutionally conserved expressed sequences.
    Bioorg Khim. 1996 Jan;22(1):48-53 (in Russian)
    PMID: 8651955

  66. Lukyanov KA, Launer GA, Tarabykin VS, Zaraisky AG, Lukyanov SA.
    Inverted terminal repeats permit the average length of amplified DNA fragments to be regulated during preparation of cDNA libraries by polymerase chain reaction.
    Anal Biochem. 1995 Aug 10;229(2):198-202
    PMID: 7485972

  67. Tarabykin VS, Lukyanov KA, Potapov VK, Lukyanov SA.
    Detection of planarian Antennapedia-like homeobox genes expressed during regeneration.
    Gene. 1995 Jun 9;158(2):197-202
    PMID: 7607541

  68. Siebert PD, Chenchik A, Kellogg DE, Lukyanov KA, Lukyanov SA.
    An improved PCR method for walking in uncloned genomic DNA.
    Nucleic Acids Res. 1995 Mar 25;23(6):1087-8
    PMID: 7731798

  69. Lukyanov S, Gurskaya NG, Lukyanov K, Tarabykin VS, Sverdlov ED.
    Highly efficient subtractive hybridisation of cDNA.
    Bioorg Khim. 1994;20:701-4 (in Russian)
    PMID: 7945464

  70. Launer GA, Lukyanov KA, Tarabykin VS, Lukyanov SA.
    Simple method for cDNA amplification starting from small amount of total RNA.
    Mol Gen Mikrobiol Virusol. 1994 Nov-Dec;(6):38-41 (in Russian)
    PMID: 7537858

  71. Lukyanov K, Tarabykin VS, Potapov VK, Bekman P, Lukyanov SA.
    The cloning of fragments of homeobox genes expressed during planarian regeneration.
    Ontogenez. 1994 Nov-Dec;25(6):28-32 (in Russian)
    PMID: 7777253