Николаев Лев Григорьевич

Доктор биологических наук


Ведущий научный сотрудник (Лаборатория структуры и функций генов человека)

Тел.: +7 (495) 3307029

Эл. почта: lev@ibch.ru

Избранные публикации

  1. Zinovyeva MV, Nikolaev LG, Kondratyeva LG, Vinogradova TV, Sverdlov ED (2018). Correlation between Expression of KLF5 and ZEB1 Transcription Factor Genes in Pancreatic Cancer. Dokl Biochem Biophys 481 (1), 219–221
  2. Abjalimov IR, Zinovyeva MV, Nikolaev LG, Kopantzeva MR, Kopantzev EP, Sverdlov ED (2017). Expression of transcription factor genes in cell lines corresponding to different stages of pancreatic cancer progression. Dokl Biochem Biophys 475 (1), 267–270
  3. Smirnov NA, Akopov SB, Didych DA, Nikolaev LG (2017). In trans promoter activation by enhancers in transient transfection. Gene 603 (0), 15–20
  4. Skvortsova YV, Kondratieva SA, Zinovyeva MV, Nikolaev LG, Azhikina TL, Gainetdinov IV (2016). Intragenic locus in human PIWIL2 gene shares promoter and enhancer functions. PLoS One 11 (6), e0156454
  5. Kotova ES, Akopov SB, Didych DA, Petrova NV, Iarovaia OV, Razin SV, Nikolaev LG (2016). Binding of protein factor CTCF within chicken genome alpha-globin locus. Acta Naturae 8 (1), 90–97
  6. Gushchanskaya ES, Artemov AV, Ulyanov SV, Logacheva MD, Penin AA, Kotova ES, Akopov SB, Nikolaev LG, Iarovaia OV, Sverdlov ED, Gavrilov AA, Razin SV (2014). The clustering of CpG islands may constitute an important determinant of the 3D organization of interphase chromosomes. Epigenetics 9 (7), 951–963
  7. Kotova ES, Akopov SB, Sverdlov ED, Nikolaev LG (2014). Transcription factor ctcf and mammalian genome organization. Biopolymers and Cell 30 (4), 260–272
  8. Kotova ES, Sorokina IV, Akopov SB, Nikolaev LG, Sverdlov ED (2013). Expression of chicken CTCF gene in COS-1 cells and partial purification of CTCF protein. Biochemistry (Mosc) 78 (8), 879–883
  9. Smirnov NA, Didych DA, Akopov SB, Nikolaev LG, Sverdlov ED (2013). Assay of insulator enhancer-blocking activity with the use of transient transfection. Biochemistry (Mosc) 78 (8), 895–903
  10. Didych DA, Shamsutdinov MF, Smirnov NA, Akopov SB, Monastyrskaya GS, Uspenskaya NY, Nikolaev LG, Sverdlov ED (2013). Human PSENEN and U2AF1L4 genes are concertedly regulated by a genuine bidirectional promoter. Gene 515 (1), 34–41
  11. Mikhailov VS, Potapov VK, Amirkhanov RN, Amirkhanov NV, Bulanenkova SS, Akopov SB, Zarytova VF, Nikolaev LG, Sverdlov ED (2013). Affinity capture of specific DNA fragments with short synthetic sequences. Russ. J. Bioorganic Chem. 39 (1), 72–76
  12. Didych DA, Smirnov NA, Kotova ES, Akopov SB, Nikolaev LG, Sverdlov ED (2011). Functional dissection of an enhancer-like element located within the second intron of the human U2AF1L4 gene. Biochemistry (Mosc) 76 (8), 951–957
  13. Sorotokina AN, Chernov IP, Stukacheva EA, Nikolaev LG, Sverdlov ED (2011). Enhancer activity of DNA fragments from the FXYD5-COX7A region of human chromosome 19. Russ. J. Bioorganic Chem. 37 (2), 217–224
  14. Bulanenkova SS, Kozlova AA, Kotova ES, Snezhkov EV, Azhikina TL, Akopov SB, Nikolaev LG, Sverdlov ED (2011). Dam methylase accessibility as an instrument for analysis of mammalian chromatin structure. Epigenetics 6 (9), 1078–1084
  15. Nikolaev LG, Akpov SB, Didych DA, Sverdlov ED (2009). Vertebrate protein CTCF and its multiple roles in a large-scale regulation of genome activity. Curr Genomics 10 (5), 294–302
  16. Didych DA, Akopov SB, Snezhkov EV, Skaptsova NV, Nikolaev LG, Sverdlov ED (2009). Identification and mapping of ten new potential insulators in the FXYD5-COX7A1 region of human chromosome 19q13.12. Biochemistry (Mosc) 74 (7), 728–733
  17. Akopov SB, Chernov IP, Wahlström T, Kostina MB, Klein G, Henriksson M, Nikolaev LG (2008). Identification of recognition sites for Myc/Max/Mxd network proteins by a whole human chromosome 19 selection strategy. Biochemistry (Mosc) 73 (11), 1260–1268
  18. Chernov IP, Stukacheva EA, Akopov SB, Didych DA, Nikolaev LG, Sverdlov ED (2008). A new technique for selective identification and mapping of enhancers within long genomic sequences. Biotechniques 44 (6), 775–784
  19. Akopov SB, Chernov IP, Vetchinova AS, Bulanenkova SS, Nikolaev LG (2007). Identification and mapping of cis-regulatory elements within long genomic sequences. Mol Biol 41 (5), 717–722
  20. Akopov SB, Chernov IP, Bulanenkova SS, Skvortsova YV, Vetchinova AS, Nikolaev LG (2007). Methods for identification of epigenetic elements in mammalian long multigenic genome sequences. Biochemistry (Mosc) 72 (6), 589–594
  21. Bulanenkova S, Snezhkov E, Nikolaev L, Sverdlov E (2007). Identification and mapping of open chromatin regions within a 140 kb polygenic locus of human chromosome 19 using E. coli Dam methylase. Genetica 130 (1), 83–92
  22. Chernov IP, Timchenko KA, Akopov SB, Nikolaev LG, Sverdlov ED (2007). Identification of tissue-specific DNA-protein binding sites by means of two-dimensional electrophoretic mobility shift assay display. Anal Biochem 364 (1), 60–66
  23. Nikolaev LG, Akopov SB, Chernov IP, Sverdlow ED (2007). Maps of cis-regulatory nodes in megabase long genome segments are an inevitable intermediate step toward whole genome functional mapping. Curr Genomics 8 (2), 137–149
  24. Nikolaev LG, Akopov SB, Chernov IP, Sverdlov ED (2007). Maps of cis-Regulatory Nodes in Megabase Long Genome Segments are an Inevitable Intermediate Step Toward Whole Genome Functional Mapping. Curr Genomics 8 (2), 137–49
  25. Shaposhnikov SA, Akopov SB, Chernov IP, Thomsen PD, Joergensen C, Collins AR, Frengen E, Nikolaev LG (2007). A map of nuclear matrix attachment regions within the breast cancer loss-of-heterozygosity region on human chromosome 16q22.1. Genomics 89 (3), 354–361
  26. Akopov SB, Ruda VM, Batrak VV, Vetchinova AS, Chernov IP, Nikolaev LG, Bode J, Sverdlov ED (2006). Identification, genome mapping, and CTCF binding of potential insulators within the FXYD5-COX7A1 locus of human Chromosome 19q13.12. Mouse Genome 17 (10), 1042–1049
  27. Vetchinova AS, Akopov SB, Chernov IP, Nikolaev LG, Sverdlov ED (2006). Two-dimensional electrophoretic mobility shift assay: Identification and mapping of transcription factor CTCF target sequences within an FXYD5-COX7A1 region of human chromosome 19. Anal Biochem 354 (1), 85–93
  28. Chernov IP, Akopov SB, Nikolaev LG, Sverdlov ED (2006). Identification and mapping of DNA binding proteins target sequences in long genomic regions by two-dimensional EMSA. Biotechniques 41 (1), 91–96
  29. Chalaya TV, Akopov SB, Nikolaev LG, Sverdlov ED (2006). Tissue specificity of methylation of cytosines in regulatory regions of four genes located in the locus FXYD5-COX7A1 of human chromosome 19: Correlation with their expression level. Biochemistry (Mosc) 71 (3), 294–299
  30. Iarovaia OV, Akopov SB, Nikolaev LG, Sverdlov ED, Razin SV (2005). Induction of transcription within chromosomal DNA loops flanked by MAR elements causes an association of loop DNA with the nuclear matrix. Nucleic Acids Res 33 (13), 4157–4163
  31. Sass AV, Ruda VM, Akopov SB, Snezhkov EV, Nikolaev LG, Sverdlov ED (2005). Regulatory potential of S/MAR elements in transient expression. Russ. J. Bioorganic Chem. 31 (1), 70–73
  32. Ruda VM, Akopov SB, Trubetskoy DO, Manuylov NL, Vetchinova AS, Zavalova LL, Nikolaev LG, Sverdlov ED (2004). Tissue specificity of enhancer and promoter activities of a HERV-K(HML-2) LTR. Virus Res 104 (1), 11–16
  33. Monastyrskaya GS, Kostina MB, Filyukova OB, Protopopova EV, Konovalova SN, Kachko AV, Nikolaev LG, Loktev VB, Sverdlov ED (2004). The transcription activation of the RIG-I gene encoding the DEXH/D protein in RH cells infected with tick-borne encephalitis virus. Russ. J. Bioorganic Chem. 30 (2), 129–132
  34. Monastyrskaia GS, Kostina MB, Filiukova OB, Protopopova EV, Konovalova SN, Kachko AV, Nikolaev LG, Loktev VB, Sverdlov ED (2004). Activation of the RIG-I gene, coding for DEXH/D-protein in infection of RH cells by tick-borne encephalitis virus. Bioorg Khim 30 (2), 146–150
  35. Chernov IP, Akopov SB, Nikolaev LG (2004). Structure and function of nuclear matrix associated regions (S/MARs). Bioorg Khim 30 (1), 3–14
  36. Chernov IP, Akopov SB, Nikolaev LG (2004). Structure and functions of nuclear matrix associated regions (S/MARs). Russ. J. Bioorganic Chem. 30 (1), 1–11
  37. Gavrilov BG, Monastyrskaia GS, Velikodvorskaia TV, Filiukova OB, Konovalova SN, Kachko AA, Protopopova EV, Nikolaev LG, Loktev VB, Sverdlov ED (2003). Late activation of interferon-induced genes IFI-54k and IFI-56k in human RH cells infected with tick-borne encephalitis virus. Bioorg Khim 29 (2), 175–180
  38. Gavrilov BG, Monastyrskaya GS, Velikodvorskaya TV, Filyukova OB, Konovalova SN, Kachko AA, Protopopova EV, Nikolaev LG, Loktev VB, Sverdlov ED (2003). Late Activation of Interferon-Induced Genes IFI-54K and IFI-56K in Human RH Cells Infected with Tick-borne Encephalitis Virus. Russ. J. Bioorganic Chem. 29 (2), 154–159
  39. Domansky AN, Akopov SB, Lebedev YB, Nikolaev LG, Sverdlov ED (2002). Enhancer activity of solitary long terminal repeat of human endogenous retrovirus K. Russ. J. Bioorganic Chem. 28 (4), 308–311
  40. Trubetskoy DO, Zavalova LL, Akopov SB, Nikolaev LG (2002). Purification of proteins specifically binding human endogenous retrovirus K long terminal repeat by affinity elution chromatography. J Chromatogr A 976 (12), 95–101
  41. Nikiforova NN, Velikodvorskaja TV, Kachko AV, Nikolaev LG, Monastyrskaya GS, Lukyanov SA, Konovalova SN, Protopopova EV, Svyatchenko VA, Kiselev NN, Loktev VB, Sverdlov ED (2002). Induction of alternatively spliced spermidine/spermine N1-acetyltransferase mRNA in the human kidney cells infected by Venezuelan equine encephalitis and tick-borne encephalitis viruses. Virology 297 (2), 163–171
  42. Domansky AN, Akopov SB, Lebedev YB, Nikolaev LG, Sverdlov ED (2002). Enhancer activity of solitary long terminal repeat of human endogenous retrovirus K. Bioorg Khim 28 (4), 345
  43. Chernov IP, Akopov SB, Nikolaev LG, Sverdlov ED (2001). Identification and mapping of nuclear matrix-attachment regions in a one megabase locus of human chromosome 19q13.12: Long-range correlation of S/MARS and gene positions. J Supramol Struct Cell Biochem 84 (3), 590–600
  44. Vinogradova TV, Leppik LP, Nikolaev LG, Akopov SB, Kleiman AM, Senyuta NB, Sverdlov ED (2001). Solitary human endogenous retroviruses-K LTRs retain transcriptional activity in Vivo, the mode of which is different in different cell types. Virology 290 (1), 83–90
  45. Bode J, Schlake T, Iber M, Schübeler D, Seibler J, Snezhkov E, Nikolaev L (2000). The Transgeneticist's Toolbox: Novel Methods for the Targeted Modification of Eukaryotic Genomes. Biol Chem 381 (910), 801–813
  46. Bogush ML, Velikodvorskaya TV, Lebedev YB, Nikolaev LG, Lukyanov SA, Fradkov AF, Pliyev BK, Boichenko MN, Usatova GN, Vorobiev AA, Andersen GL, Sverdlov ED (1999). Identification and localization of differences between Escherichia coli and Salmonella typhimurium genomes by suppressive subtractive hybridization. Mol Gen Genet 262 (45), 721–729
  47. Chernov IP, Polyakov AS, Akopov SB, Nikolaev LG, Azhikina TL, Kostina MB, Sverdlov ED (1999). Identification and human chromosome 19 mapping of a chromosome-specific repeated element binding preferentially to nuclear matrix. Russ. J. Bioorganic Chem. 25 (4), 242–247
  48. Chernov IP, Polyakov AS, Akopov SB, Nikolaev LG, Azhikina TL, Kostina MB, Sverdlov ED (1999). Identification and human chromosome 19 mapping of a chromosome-specific repeated element binding preferentially to nuclear matrix. Bioorg Khim 25 (4), 281
  49. Akopov SB, Nikolaev LG, Khil PP, Lebedev YB, Sverdlov ED (1998). Long terminal repeats of human endogenous retrovirus K family (HERV-K) specifically bind host cell nuclear proteins. FEBS Lett 421 (3), 229–233
  50. Akopov SB, Nikolaev LG, Tyrsin OY, Ruzov AS, Sverdlov ED (1997). Identification and Characterization of 14 Sequences from the Chinese Hamster Genome Preferentially Binding to the Nuclear Matrix. Bioorg Khim 23 (9), 731
  51. Akopov SB, Nikolaev LG, Tyrsin OY, Ruzov AS, Sverdlov ED (1997). Identification and characterization of 14 sequences from the Chinese hamster genome preferentially binding to the nuclear matrix. Russ. J. Bioorganic Chem. 23 (9), 659–663
  52. Nikolaev LG (1996). Identification and purification of two proteins that recognize the enhancer sequence of human immumodeficiency virus type 1. Mol Biol 30 (32), 425–428
  53. Nikolaev LG, Tsevegiyn T, Akopov SB, Ashworth LK, Sverdlov ED (1996). Construction of a chromosome specific library of human MARs and mapping of matrix attachment regions on human chromosome 19. Nucleic Acids Res 24 (7), 1330–1336
  54. Volik S, Lebedev Y, Nikolaev L, Shevchenko Y, Vinogradova T, Kopantzev E, Kolesnik T, Monastyrskaya G, Kunz U, Grzeschik KH, Ashworth LK, Lennon G, Sverdlov E (1995). Mapping of transcribed sequences on human chromosome 19. Mitochondrial DNA 6 (1), 13–26