Nucleic Acids Res, 1999, 27(6):1558-1560

Amplification of cDNA ends based on template-switching effect and step-out PCR

A new method for amplifying cDNA ends is described which requires only first-strand cDNA synthesis and a single PCR to generate a correct product with very low or no background. The method can be successfully applied to total RNA as well as poly A+ RNA. The same first-strand cDNA can be used to amplify flanking sequences of any cDNA species present in the sample.

Matz M, Shagin D, Bogdanova E, Britanova O, Lukyanov S, Diatchenko L, Chenchik A

Лаборатория генетически кодируемых молекулярных инструментов, Лаборатория методов иммуносеквенирования, Группа структурной организации Т-клеточного иммунитета, Отдел геномики и постгеномных технологий

: 10.1093/nar/27.6.1558
Scopus: 2-s2.0-0033559113
: 10037822
IBCH: 1923
Ссылка на статью в журнале: https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/27.6.1558
Кол-во цитирований на 03.2024: 336
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