Cloning of the ha-SDGF gene cDNA from a highly metastatic golden hamster cell line by subtractive hybridization
Using subtractive hybridization, a cDNA library containing over 50% of clones specific for a highly metastatic cell line was obtained from two hamster embryo fibroblast lines with different metastatic potentials. Most of the clones (83%) contained new sequences. One clone contained the ha-SDGF gene cDNA homologous to SDGF cDNA from rodents. The level of ha-SDGF mRNA expression was considerably higher in the highly metastatic cell line. © 1996 MAEe cyrillic signK Hayκa/Interperiodica Publishing.