The cointegration rate into the aspen and birch genomes of foreign genes from a binary vector and a disarmed Ti plasmid pCBE21 carried by the same Agrobacterium tumefaciens strain was studied. The cotransformation rate for the genes within the Ti plasmid varied from 30 to 100%; while the transformation rate for the gene from TLregion was twofold higher as compared with the TRregion. On the average, the gene transfer from all three T-DNAs was recorded in 10. 9% of the transgenic lines. For the vector pBI121, the cotransformation rates for the genes from both regions of pCBE21 T-DNA were higher as compared with the vector pGS. In addition, a concurrent transfer of the genes from the Ti plasmid TLand TRregions was recorded only after the transformation with the vector pBI121. These results can be used for constructing woody plants containing several genes. © 2010 Pleiades Publishing, Ltd.