In this work a primary structural analysis has been carried out and biological properties of protein-peptide extract from lamb's quarters (Chenopodium album L.) were studied. It has been shown that the obtained enriched concentrate displayed potent fungicidal properties towards a number of phytopathogenic (Fusarium oxysporum, Thielaviopsisbasicola) and opportunistic fungi (Aspergillus spp.) at a quantitative level comparable with the effect of commercial antifungal drugs, at the same time possessing moderate antibacterial properties (Escherichiacoli, Bacillussubtilus) not associated with ribosome-inactivating activity. When studying the analgesic activity of this extract in acute pain models (hot water∗, hot plate∗, and electro stimulation∗), it demonstrated activity in a wide range of active concentrations and at the same time their effect was manifested both at the spinal and supraspinal levels of pain sensitivity. The primary structural characteristic of the total fractions of the active extract based on mass spectrometry analysis revealed a set of compounds in a wide range of molecular masses (1.9-12.5 kDa), and protein chemical base of some of them has been confirmed by a combination of spectrophotometric analysis and N-terminal Edman sequencing. The totality of the data obtained allows positioning of the lamb's quarters' seeds as a rich source of biologically active polypeptides with antibiotic and analgesic properties.