Russ. J. Bioorganic Chem., 1996, 22(7):428-433

Bacterial synthesis of human calcitonin and miniproinsulin as fusion proteins with the corresponding antisense peptides and metal-binding peptide

To verify experimentally the molecular recognition theory, plasmids were constructed that provided the efficient synthesis of hybrid proteins composed of human calcitonin or miniproinsulin, the corresponding antisense peptides, and a histidine-rich metal-binding peptide. A method for isolation of the hybrid proteins by metal-chelating chromatography, cleavage, and renaturation was developed. © 1996 MAEe cyrillic signK Hayκa/Interperiodica Publishing.

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