Mechanism of pepsin catalysis: General base catalysis by the active site carboxylate ion
A workable procedure for cross-linking of a protein crystal - acid protease from Endothia parasitica - in 2.7 M ammonium sulfate solution with bifunctional reagent glutaraldehyde has been found. Using the cross-linking technique, we have made one good quality heavy atom derivative of uranyl into three or even more isomorphous derivatives for this particular protein. © 1978.