Recent works have demonstrated the potential of fluorescent protein chromophores as useful dyes with unique photophysical properties. Herein, we report the synthesis of two novel highly fluorescent dyes based on conformationally locked chromophores of the fluorescent proteins CFP and Sirius. These novel synthetic dyes demonstrate high fluorescence quantum yields and pH-independence in the physiological pH range, making them promising molecular tools for a broad range of fluorescent labeling applications.