Education

Period	Coyntry, city	University	Additional info
2010–2012	Russia, Nizhny Novgorod	Lobachevsky State University of Nizhny Novgorod	MSc in Radiophysics
2006–2010	Russia, Nizhny Novgorod	Lobachevsky State University of Nizhny Novgorod	BSc in Radiophysics

Work experience

Period	Coyntry, city	Employer	Additional info

IBCh positions

2019–2023	Junior research fellow	Laboratory of Extrasynaptic Signalling
2018–2022	Junior research fellow	Laboratory of optical bioimaging

IBCh memberships

Scientific interests

neuronal-astrocytic interaction, extrasynaptic transmission, extracellular matrix, glutamate transporters, astrocytic-vascular interaction, methods in neuroscience

Scientific results

List of works:

peer-reviewed publications:

• Christie I.N., Theparambil S.M., Braga A., Doronin M., Hosford P.S., Brazhe A., Mascarenhas A., Nizari S., Hadjihambi A., Wells J.A., Hobbs A., Semyanov A., Abramov A.Y., Angelova P.R., Gourine A.V. Astrocytes produce nitric oxide via nitrite reduction in mitochondria to regulate cerebral blood flow during brain hypoxia. Cell Reports 42 (12): 113514 (2023) doi: 10.1016/j.celrep.2023.113514
• Fedotova A., Brazhe A., Doronin M., Toptunov D., Pryazhnikov E., Khiroug L., Verkhratsky A., Semyanov A. Dissociation Between Neuronal and Astrocytic Calcium Activity in Response to Locomotion in Mice. Function 4 (4), zqad019 (2023) doi: 10.1093/function/zqad019
• Dembitskaya Y., Gavrilov N., Kraev I., Doronin M., Tang Y., Li L., Semyanov A. Attenuation of the extracellular matrix increases the number of synapses but suppresses synaptic plasticity through upregulation of SK channels. Cell Calcium 96: 102406 (2021) doi: 10.1016/j.ceca.2021.102406;
• Marina N., Christie I.N., Korsak A., Doronin M., Brazhe A., Hosford P.S., Wells J.A., Sheikhbahaei S., Humoud I., Paton J.F.R., Lythgoe M.F., Semyanov A., Kasparov S., Gourine A.V. Astrocytes monitor cerebral perfusion and control systemic circulation to maintain brain blood flow. Nature Communications 11 (1): 131 (2020) doi: 10.1038/s41467-019-13956-y;
• Brazhe A.R., Doronin M.S., Popov A.V., Denisov P.A., Semyanov A.V. Research of calcium dynamics patterns into brain astrocytic networks. Sechenov Russian physiological journal 105 (11): 1436-1451 (2019) doi: 10.1134/S0869813919110037
• Popov A.V., Kushnireva L.A., Doronin M.S., Henley J.M. Kainate receptors are the key to understanding of synaptic plasticity, learning and memory. Modern Technologies in Medicine 9 (4): 228-238 (2017) doi: 10.17691/stm2017.9.4.28;
• Doronin M., Popov A., Makovkin S., Dembitskaya Yu., Semyanov A. Software development for custom-made two-photon system for implications on neuroscience. Opera Medica et Physiologica 3 (S1): 7-8 (2017) doi: 10.20388/omp2017.00s1.001;
• Doronin M., Makovkin S., Popov A. Development of self-made LSM software using in neuroscience. IOP Conference Series: Materials Science and Engineering 224 (1): 012057 (2017) doi: 10.1088/1757-899X/224/1/012057;
• Doronin M., Popov A. Development and design of up-to-date laser scanning two-photon microscope using in neuroscience. Proceedings of SPIE – The International Society for Optical Engineering 10250: 102501M (2017) doi: 10.1117/12.2267160;
• Doronin M., Popov A. Design and building of modern two-photon laser scanning microscope for using in neuroscience. MATEC Web of Conferences 77: 11002 (2016) doi: 10.1051/matecconf/20167711002;
• Doronin M.S., Popov A.V. Development and design of advanced two-photon microscope used in neuroscience. Journal of Physics: Conference Series 741 (1): 012070 (2016) doi: 10.1088/1742-6596/741/1/012070;
• Doronin M.S., Dembitskaya Yu.V., Semyanov A.V. Up- and down-regulation of h-channels conductance in CA1 hippocampal neurons. Opera Medica et Physiologica Issue Supplement S2: 121 (2016) doi: 10.20388/OMP2016.00S2.006;
• Popov A.V., Doronin M.S., Dembitskaya Y.V., Semyanov A.V. The implementation of cost-effective and adaptive two-photon microscope for neuroscience. Opera Medica et Physiologica Issue Supplement S2: 117 (2016) doi: 10.20388/OMP2016.00S2.006;
• Popov A., Doronin M. Design, upgrading and creation of the state-of-the-art two-photon laser microscope view of features in Neuroscience. 6th International Workshop on Computer Science and Engineering, WCSE 2016 pp. 673-677 (2016);
• Doronin M.S., Popov A.V.. Analysis, Modification And Development Of The State-Of-The-Art Two-Photon Laser Microscope In View Of The Features Of Its Use In Neurosciene. Opera Medica et Physiologica 1 (1) 8-9 (2015) doi: 10.20388/omp2015.00s1.0012;
• Wlodarczyk A.I., Xu C., Song I., Doronin M., Wu Y.-W., Walker M., Semyanov A. Tonic GABA_A conductance decreases membrane time constant and increases EPSP-spike precision in hippocampal pyramidal neurons. Front Neural Circuits 7: 205 (2013) doi: 10.3389/fncir.2013.00205;

proceedings of the conferences:

• Doronin M.S., Dembitskaya Yu.V., Semyanov A.V. Up- and down-regulation of h-channels conductance in CA1 hippocampal neurons. Proceedings of the 71^st International school-conference of the young scientists “Biosystems: organization, behavior, management” (Lobachevsky State University of Nizhny Novgorod, Russia, 17.04.2018–20.04.2018);
• Makovkin S.Yu., Doronin M.S., Popov A.V. Two-photon laser scanning microscopy system. Proceedings of the XV International conference NIDays 2016 (Moscow, Russia, 25.11.2016);
• Doronin M., Popov A. Development of modern two-photon microscope in use of neuroscience. Proceedings of the International symposium IEEE “Video and Audio Signal Processing in the Context of Neurotechnologies (SPCN 2016)” (State University of Saint-Petersburg, Saint-Petersburg, Russia, 30.06.2016–02.07.2016);
• Doronin M.S., Popov A.V. Development of modern two-photon microscope in use of neuroscience. Proceedings of the 69^th International school-conference of the young scientists “Biosystems: organization, behavior, management” (Lobachevsky State University of Nizhny Novgorod, Russia, 27.04.2016–29.04.2016);
• Doronin M.S., Popov A.V. Development and design of advanced two-photon microscope used in neuroscience. Proceedings of the 3^rd International school and conference on optoelectronics, photonics, engineering and nanosctructures “Saint Petersburg OPEN 2016” (State University of Saint-Petersburg, Saint-Petersburg, Russia, 28.03.2016–30.03.2016);
• Doronin M.S. Up- and down-regulation of h-channels conductance in CA1 hippocampal neurons of rats and mice. Proceedings of the 19^th Session of the young scientists of the Nizhny Novgorod region (natural and mathematical sciences) (sanatorium “Morozovsky”, Nizhny Novgorod region, Russia, 27.05.2014–30.05.2014);

patents:

• Software patent “High precision control program for IR femtosecond laser wavelength "ChangeLaserWL"” (certificate of registration №2021660990 dated by 05.07.2021);
• Software patent “High-precision control program for the intensity and angle of deflection of the IR laser beam "ControlByAOM"” (certificate of registration №2021660704 dated by 30.06.2021);
• Software patent “High-precision control program for focusing positioner "FocusByStepper"” (certificate of registration №2021613239 dated by 04.03.2021);
• Software patent “High-precision control program for two-axis sample positioner "GoByRails"” (certificate of registration №2020610809 dated by 20.01.2020);
• Software patent “Two-photon scanning microscope control program "AMAscan"” (certificate of registration №2020610281 dated by 13.01.2020);
• Know-how “Universal module for intensity regulation of homogeneous light path delivering to specimen” (order №589-OD dated by 29.12.2018);
• Know-how “High-accurate focusing system of two-photon laser scanning microscope” (order №139 dated by 27.12.2018);
• Know-how “Universal high-effective collimator of laser two-photon scanning microscope for delivering and homogeneous illumination of light path to specimen” (order №622-OD dated by 28.12.2017);
• Know-how “Innovative placement of detectors of laser two-photon scanning microscope” (order №556-OD dated by 28.12.2016);
• Utility model “Two-photon scanning microscope” (patent №162395 dated by 18.05.2016);

tutorials:

• Doronin M., Semyanov A. Application of the opto- and chemogenetic viral constructs for in vivo and in vitro research. Tutorial for students of Lomonosov Moscow State University and for PhD students of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS (2018);
• Doronin M., Semyanov A. Immunohistochemical evaluation of proteins expression encoded by viral vectors in the animal brain. Tutorial for students of Lomonosov Moscow State University and for PhD students of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS (2018);
• Doronin M., Dembitskaya Yu., Lebedeva A., Tyurikova O., Semyanov A. Analysis of electrophysiological data in Neuroscience. Tutorial for students and PhD students of Lobachevcky State University of Nizhny Novgorod (2015);
• Tyurikova O., Dembitskaya Yu., Doronin M., Lebedeva A., Babaev A., Semyanov A. PCR-based genotyping of genetically modified animals using the PCR method (in Russian). Tutorial for students and PhD students of Lobachevcky State University of Nizhny Novgorod (2014).