Barnase*Barstar-guided two-step targeting approach for drug delivery to tumor cells in vivo

A team of scientists from the Laboratory of molecular immunology and Nursery for laboratory animals in collaboration with colleagues from other Russian institutes for the first time proposed the use of Barnase-Barstar pair as a «molecular glue». Targeting was mediated through the use of a scaffold protein DARPin_9–29 specific for the human epidermal receptor 2 (HER2) antigen that is highly expressed on some types of cancer and Barnase*Barstar native bacterial proteins interacted with each other with Kd 10-14 M. The results obtained indicate that the new system combining DARPin and Barnase*Barstar can be useful both for the drug development and for monitoring the response to treatment in vivo in preclinical studiesаа. This work was published in the Journal of Controlled Release. Learn more

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