We analysed spatiotemporal properties of Ca signals in protoplasmic astrocytes in the CA1 stratum radiatum of hippocampal slices from young (2–3 months old) mice housed in control conditions or exposed to a caloric restriction (CR) diet for one month. The astrocytic Ca events became shorter in duration and smaller in size; they also demonstrated reduced velocity of expansion and shrinkage following CR. At the same time, Ca signals in the astrocytes from the CR animals demonstrated higher amplitude and the faster rise and decay rates. These changes can be attributed to CR-induced morphological remodelling and uncoupling of astrocytes described in our previous study. CR-induced changes in the parameters of Ca activity were partially reversed by inhibition of gap junctions/hemichannels with carbenoxolone (CBX). The effect of CBX on Ca activity in CR-animals was unexpected because the diet already decreases gap junctional coupling in astrocytic syncytia. It may reflect the blockade of hemichannels also sensitive to this drug. Thus, CR-induced morphological remodelling of astrocytes is at least partly responsible for changes in the pattern of Ca activity in the astrocytic network. How such changes in spatiotemporal Ca landscape can translate into astrocytic physiology and neuron-glia interactions remains a matter for future studies. 2+ 2+ 2+ 2+ 2+ 2+ 2+