Objective: This study continues our investigation into the mechanisms that control early developmental gene expression in Xenopus laevis frog embryos. Previous findings indicated that maternal mRNAs of two crucial genes, the nuclear retinoic acid receptor Rxrg and the pluripotency factor Pou5f3, form complexes with the ribonucleoprotein Ybx1, thereby increasing their stability. As a result of this work, we have discovered that the stability of maternal mRNA ssx2ip (coding for a conserved protein known as Msd1 or ADIP, a key player in centrosome maturation) is dependent on Ybx1. Methods: Initially, we assessed the impact of altering Ybx1 function and verified the specificity of the results through rescue experiments. Subsequently, we ruled out the potential activation of ssx2ip gene expression by Ybx1, providing evidence for an elevation in its mRNA levels even under conditions of complete transcription suppression. As a final step, we examined Ybx1’s ability to interact directly with ssx2ip mRNA by performing a Ribo Immuno Precipitation assay (RIP). Results and Discussion: Our investigation revealed that Ybx1 plays a critical role in maintaining the stability of maternal mRNA ssx2ip (synovial sarcoma X breakpoint 2 interacting protein). Specifically, we observed the formation of a ribonucleoprotein complex between Ybx1 and ssx2ip mRNA, with a notable involvement of Ybx1’s cold shock domain (CSD). Conclusions: This study supports our hypothesis concerning Ybx1’s preferential binding to maternal transcripts, opening up new avenues for identifying potential cis motifs recognized by trans-acting regulators such as Ybx1. These findings are significant for unraveling novel mechanisms of gene expression control during the early stages of development.