Neutrophils can suppress eosinophilia, but in allergic airway inflammation, the switching from neutrophil‐ to eosinophil‐mediated response occurs. To investigate the mechanisms of such switching we analyzed the kinetics of eosinophil and neutrophil proportions in blood samples of mice during the course of allergen application. Mice received five oropharyngeal applications of Aspergillus fumigatus extract in a dose of 7 μg per mouse every 72 hours. Blood samples were collected before each allergen application. Neutrophils and eosinophils were detected by both flow cytometry and differential cell count of cytospins. By flow cytometry myeloid cells were identified as CD45⁺CD11b⁺CD172⁺. Neutrophils among myeloid cells were identified as Ly6G⁺SiglecF⁻, eosinophils as Ly6GlowSiglecF⁺. Significant eleva